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“魔角自旋交换极化反转”与“标记丙氨酸的几何分析”方法用于跨膜螺旋排列的比较。

Comparison of "Polarization inversion with spin exchange at magic angle" and "geometric analysis of labeled alanines" methods for transmembrane helix alignment.

作者信息

Vostrikov Vitaly V, Grant Christopher V, Daily Anna E, Opella Stanley J, Koeppe Roger E

机构信息

Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, USA.

出版信息

J Am Chem Soc. 2008 Sep 24;130(38):12584-5. doi: 10.1021/ja803734k. Epub 2008 Sep 3.

DOI:10.1021/ja803734k
PMID:18763771
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2609751/
Abstract

Using the model alpha-helical peptide acetyl-GGALW5LALALALALALALW19LAGA-ethanolamide ("GWALP23"), we have compared the polarization inversion with spin exchange at magic angle method and geometric analysis of labeled alanines method for estimating the transmembrane helix orientation. For GWALP23 in bilayers of a short lipid, dilauroylphosphatidylcholine, we find general agreement between the two methods, with a static helix tilt of about 11degrees-13degrees with respect to the bilayer normal.

摘要

使用模型α-螺旋肽乙酰基-GGALW5LALALALALALALW19LAGA-乙醇酰胺（“GWALP23”），我们比较了用于估计跨膜螺旋取向的魔角自旋交换极化反转方法和标记丙氨酸的几何分析方法。对于短链脂质二月桂酰磷脂酰胆碱双层中的GWALP23，我们发现这两种方法总体上是一致的，相对于双层法线，静态螺旋倾斜约11度至13度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/160a/2609751/c2393b45363e/nihms-81908-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/160a/2609751/c1331a1fb1cb/nihms-81908-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/160a/2609751/0f7fcd29feb6/nihms-81908-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/160a/2609751/c2393b45363e/nihms-81908-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/160a/2609751/c1331a1fb1cb/nihms-81908-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/160a/2609751/0f7fcd29feb6/nihms-81908-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/160a/2609751/c2393b45363e/nihms-81908-f0003.jpg

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Helical distortion in tryptophan- and lysine-anchored membrane-spanning alpha-helices as a function of hydrophobic mismatch: a solid-state deuterium NMR investigation using the geometric analysis of labeled alanines method.色氨酸和赖氨酸锚定的跨膜α螺旋中螺旋扭曲与疏水错配的关系：使用标记丙氨酸几何分析法的固态氘核磁共振研究
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