Haffajee A D, Teles R P, Patel M R, Song X, Yaskell T, Socransky S S
Department of Periodontology, The Forsyth Institute, Boston, MA 02115, USA.
J Periodontal Res. 2009 Aug;44(4):520-8. doi: 10.1111/j.1600-0765.2008.01155.x. Epub 2008 Oct 7.
Little is known regarding the factors that affect the microbial composition of supragingival biofilms. This study was designed to test the hypothesis that tooth location affects the microbial composition of supragingival plaque beyond the effect due to plaque mass as reflected by total DNA probe count.
Supragingival plaque samples were taken from the mesiobuccal aspect of each tooth in 187 subjects (n = 4745 samples). All samples were individually analyzed for their content of 40 bacterial species using checkerboard DNA-DNA hybridization. Significance of differences in mean species counts and proportions were determined among tooth surfaces and six tooth type categories: molars, bicuspids, incisors/canines in the mandible and maxilla separately using the Kruskal-Wallis test. Stepwise multiple linear regression was employed to examine the relationship between species proportions and total DNA probe count, tooth location, periodontal and smoking status, age and sex.
All species differed significantly among tooth types and among the six tooth categories. Higher plaque levels were seen on molars and lower incisors. Some differences observed between tooth types could be partly explained by the level of plaque. Teeth with high plaque mass exhibited high levels of Capnocytophaga gingivalis, Actinomyces naeslundii genospecies 2, Campylobacter rectus and Campylobacter showae. However, certain species, such as Veillonella parvula and Streptococcus sanguinis, differed significantly at different tooth locations despite similarities in plaque mass. Twenty of the test species exhibited a significant association with tooth location after adjusting for total DNA probe count and subject level factors.
While plaque mass was associated with differences in proportions of many species in supragingival biofilms, tooth location also was strongly associated with species proportions in both univariate and multivariate analyses.
关于影响龈上生物膜微生物组成的因素，人们了解甚少。本研究旨在验证这一假说：牙齿位置对龈上菌斑微生物组成的影响，超出了总DNA探针计数所反映的菌斑量的影响。
从187名受试者的每颗牙齿的近中颊面采集龈上菌斑样本（n = 4745个样本）。使用棋盘式DNA-DNA杂交技术，对所有样本中的40种细菌的含量进行单独分析。分别使用Kruskal-Wallis检验，确定牙齿表面和六类牙齿类型（磨牙、双尖牙、下颌和上颌的切牙/尖牙）之间平均菌种计数和比例差异的显著性。采用逐步多元线性回归分析，研究菌种比例与总DNA探针计数、牙齿位置、牙周状况、吸烟状况、年龄和性别的关系。
所有菌种在牙齿类型和六类牙齿之间均存在显著差异。磨牙和下切牙的菌斑水平较高。牙齿类型之间观察到的一些差异，部分可由菌斑水平来解释。菌斑量高的牙齿，牙龈二氧化碳嗜纤维菌、内氏放线菌基因种2、直肠弯曲菌和昭和弯曲菌的水平较高。然而，尽管菌斑量相似，但某些菌种，如小韦荣球菌和血链球菌，在不同牙齿位置存在显著差异。在调整总DNA探针计数和个体水平因素后，20种受试菌种与牙齿位置存在显著关联。
虽然菌斑量与龈上生物膜中许多菌种比例的差异有关，但在单变量和多变量分析中，牙齿位置也与菌种比例密切相关。
