Department of Internal Medicine, Renmin Hospital of Wuhan University, 430060, Wuhan, China.
J Mol Histol. 2009 Aug;40(4):261-8. doi: 10.1007/s10735-009-9237-y. Epub 2009 Nov 12.
Luminescent semiconductor quantum dots (QDs) are a new class of fluorescent label with wide ranges of applications in cell imaging. In this study, we evaluated the capability of QDs immunofluorescence histochemistry (QDs-IHC) for detecting antigens of caveolin-1 and PCNA in the lung cancer tissue microarray (TMA) in comparison with the conventional immunohistochemistry (IHC) technique. Both methods revealed consistent antigen localization and statistically non-significant detection rates of caveolin-1 and PCNA expressions in our study. However, the sensitivity of QDs-IHC was higher than IHC. The positive detection rates of caveolin-1 and PCNA by QDs-IHC were 57% (40/70) and 86% (60/70), respectively, which were higher than the detection rates of 47% (33/70) and 77% (54/70), respectively, by IHC. Moreover, QDs exhibited a much better photostability, a broader excitation spectrum and a longer fluorescence lifetime. We showed here the advantages of QDs-IHC over IHC for the detection of caveolin-1 and PCNA in lung cancer TMA.
发光半导体量子点（QDs）是一类新型荧光标记物，在细胞成像中有广泛的应用。在本研究中，我们将 QDs 免疫荧光组织化学（QDs-IHC）与传统免疫组织化学（IHC）技术进行了比较，评估了其在肺癌组织微阵列（TMA）中检测小窝蛋白-1 和 PCNA 抗原的能力。两种方法在本研究中均显示出一致的抗原定位和统计学上无显著差异的小窝蛋白-1 和 PCNA 表达检测率。然而，QDs-IHC 的灵敏度高于 IHC。QDs-IHC 对小窝蛋白-1 和 PCNA 的阳性检出率分别为 57%（40/70）和 86%（60/70），高于 IHC 的 47%（33/70）和 77%（54/70）。此外，QDs 具有更好的光稳定性、更宽的激发光谱和更长的荧光寿命。我们在这里展示了 QDs-IHC 在检测肺癌 TMA 中小窝蛋白-1 和 PCNA 方面优于 IHC 的优势。
