Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Ilan 262, Taiwan.
Fish Shellfish Immunol. 2010 Apr;28(4):542-8. doi: 10.1016/j.fsi.2009.12.013. Epub 2010 Jan 1.
We examined the physiological (hemolymph glucose, lactate, and lipid) and innate non-specific immune responses (total hemocyte count (THC), phenoloxidase (PO) activity, respiratory bursts (release of superoxide anion, O(2)(-)) and superoxide dismutase (SOD) activity) to white spot syndrome virus (WSSV) in white shrimp (Litopenaeus vannamei) that were individually injected with 0.1, 0.5, and 1 ng g(-1) ferritin. Results showed that the THC, PO activity, and respiratory bursts of L. vannamei obviously increased (p < 0.05) 12 h after being injected with any dose of ferritin. However, the THC, PO activity, and respiratory bursts of L. vannamei that had received 0.5 and 1 ng g(-1) ferritin were significant higher than those of the other groups at 36-60, 60-72, and 36-60 h, respectively. SOD activities of L. vannamei 12 h after receiving 0.1, 0.5, and 1 ng g(-1) ferritin were significantly higher than those receiving saline. L. vannamei injected with ferritin at any dose maintained lower glucose, lactate, and lipid levels in response to WSSV challenge after 12-36, 24-48, and 36-60 h, respectively. The survival of shrimp that had received 0.5 and 1 ng g(-1) ferritin was significantly higher than that of shrimp that received saline and of control shrimp after 72 h. The ferritin messenger RNA transcripts of shrimp that had received 0.5 and 1 ng g(-1) ferritin were significantly higher than that of shrimp that received saline after 36 h. It was, therefore, concluded that the immune ability and resistance against WSSV infection increased in L. vannamei that had received > 0.5 ng g(-1) ferritin. Ferritin does play important roles in the innate immunity of the white shrimp. We observed higher SOD activities of L. vannamei that had received 0.1, 0.5, and 1 ng ferritin after 12 h than those that had received only saline (control), and the high SOD expression remained at the same levels even after 72 h of treatment.
我们检测了生理（血淋巴葡萄糖、乳酸和脂质）和先天非特异性免疫应答（总血细胞计数（THC）、酚氧化酶（PO）活性、呼吸爆发（超氧阴离子释放，O(2)(-))和超氧化物歧化酶（SOD）活性）对白斑综合征病毒（WSSV）的影响，这些虾单独注射了 0.1、0.5 和 1ng g(-1) 的铁蛋白。结果表明，在注射任何剂量铁蛋白后 12 小时，凡纳滨对虾（Litopenaeus vannamei）的 THC、PO 活性和呼吸爆发明显增加（p < 0.05）。然而，在 36-60、60-72 和 36-60 小时，接受 0.5 和 1ng g(-1) 铁蛋白的凡纳滨对虾的 THC、PO 活性和呼吸爆发均显著高于其他组。在接受 0.1、0.5 和 1ng g(-1) 铁蛋白 12 小时后，凡纳滨对虾的 SOD 活性均显著高于接受生理盐水的组。凡纳滨对虾在接受任何剂量铁蛋白后 12-36、24-48 和 36-60 小时对 WSSV 挑战的葡萄糖、乳酸和脂质水平保持较低水平。在 72 小时后，接受 0.5 和 1ng g(-1) 铁蛋白的虾的存活率明显高于接受生理盐水的虾和对照虾。在 36 小时后，接受 0.5 和 1ng g(-1) 铁蛋白的虾的铁蛋白信使 RNA 转录物明显高于接受生理盐水的虾。因此，我们得出结论，在凡纳滨对虾中，当铁蛋白的浓度>0.5ng g(-1)时，其免疫能力和对 WSSV 感染的抵抗力都会增强。铁蛋白在凡纳滨对虾的先天免疫中起着重要作用。我们观察到，在接受 0.1、0.5 和 1ng 铁蛋白 12 小时后，凡纳滨对虾的 SOD 活性高于仅接受生理盐水（对照）的虾，并且即使在处理 72 小时后，SOD 表达仍保持在相同水平。
