Telejko B, Kuzmicki M, Zonenberg A, Modzelewska A, Niedziolko-Bagniuk K, Ponurkiewicz A, Wawrusiewicz-Kurylonek N, Nikolajuk A, Szamatowicz J, Laudanski P, Kretowski A, Gorska M
Department of Endocrinology, Diabetology and Internal Medicine, Medical University of Bialystok, 15 - 276 Bia ł ystok Poland.
Exp Clin Endocrinol Diabetes. 2010 Feb;118(2):87-92. doi: 10.1055/s-0029-1238313. Epub 2010 Feb 23.
OBJECTIVES: We studied the effect of an oral glucose load on circulating ghrelin, as well as ghrelin and ghrelin receptor (GHS-R1a) mRNA expression in subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT) and placental tissue from pregnant women with gestational diabetes (GDM) and normal glucose tolerance (NGT). METHODS: Plasma total ghrelin levels were measured in 58 patients with GDM and 61 women with NGT by radioimmunoassay. Ghrelin and GHS-R1a mRNA expression was studied in 16 subjects with GDM and 20 healthy pregnant women at term, using RT-PCR. RESULTS: Basal ghrelin concentrations and the maximal decrease in ghrelin levels after glucose load did not differ in the women with GDM and NGT (399.1 [299.6-563.3] pg/ml vs. 400.9 [302.3-475.8] pg/ml and 127.6 [23.1-213.1] pg/ml vs. 101.7 [44.0-217.6] pg/ml, respectively). Ghrelin mRNA expression in placental tissue was significantly higher in the subjects with GDM than in the healthy pregnant women (0.06 [0.03-0.07] AU vs. 0.02 [0.015-0.03 AU], p=0.02), whereas GHS-R1a mRNA expression in all three tissues studied did not differ between the two groups. Multiple regression analysis revealed that ghrelin mRNA expression in SAT was significantly predicted by serum insulin (beta=0.62, p=0.01), explaining 42% of its variability. CONCLUSIONS: Ghrelin mRNA expression in placental tissue was higher in the GDM than in NGT subjects, whereas no association between circulating ghrelin and GDM was observed.
目的：我们研究了口服葡萄糖负荷对循环中胃饥饿素的影响，以及患有妊娠期糖尿病（GDM）和糖耐量正常（NGT）的孕妇皮下脂肪组织（SAT）、内脏脂肪组织（VAT）和胎盘组织中胃饥饿素及胃饥饿素受体（GHS-R1a）mRNA表达的影响。 方法：采用放射免疫分析法测定58例GDM患者和61例NGT女性的血浆总胃饥饿素水平。运用逆转录聚合酶链反应（RT-PCR）研究16例GDM患者和20例足月健康孕妇的胃饥饿素和GHS-R1a mRNA表达。 结果：GDM组和NGT组女性的基础胃饥饿素浓度以及葡萄糖负荷后胃饥饿素水平的最大降幅无差异（分别为399.1[299.6 - 563.3] pg/ml对400.9[302.3 - 475.8] pg/ml和127.6[23.1 - 213.1] pg/ml对101.7[44.0 - 217.6] pg/ml）。GDM患者胎盘组织中胃饥饿素mRNA表达显著高于健康孕妇（0.06[0.03 - 0.07] AU对0.02[0.015 - 0.03] AU，p = 0.02），而在所有研究的三种组织中，两组间GHS-R1a mRNA表达无差异。多元回归分析显示，血清胰岛素可显著预测SAT中胃饥饿素mRNA表达（β = 0.62，p = 0.01），解释其变异性的42%。 结论：GDM患者胎盘组织中胃饥饿素mRNA表达高于NGT患者，而未观察到循环胃饥饿素与GDM之间存在关联。
Zotero 插件