Department of Pharmaceutical Technology, Institute of Pharmacy, Leopold-Franzens-University Innsbruck, Innsbruck, Austria.
J Drug Target. 2011 Aug;19(7):562-72. doi: 10.3109/1061186X.2010.542243. Epub 2010 Dec 22.
The purpose of this study was to develop and characterize new surface-modified iron oxide nanoparticles demonstrating the efficiency to be internalized by human endothelial progenitor cells (EPCs) from umbilical cord blood.
Iron oxide nanoparticles were coated with polyacrylic acid-cysteine (PAA-Cys) by either in situ precipitation or postsynthesis. The nanoparticles were characterized by X-ray powder diffraction. EPCs were labeled with PAA-Cys-modified iron oxide nanoparticles or with uncoated nanoparticles. The relaxivity of uncoated and coated iron oxide nanoparticles as well as EPCs labeled with PAA-Cys-modified iron oxide were determined.
Addition of PAA-Cys increased the particle size from 10.4 to 144 and 197 nm, respectively. The X-ray powder diffraction pattern revealed that the particles consist of Fe(3)O(4) with a spinal structure. Postsynthesis coated particles showed a cellular uptake of 85% and 15.26 pg iron/cell. For both types of particles the relaxivity ratio was at least 2-fold higher than that of the gold standard Resovist(®).
The PAA-Cys coated iron oxide nanoparticles are a promising tool for labeling living cells such as stem cells for diagnostic and therapeutic application in cell-based therapies due to their high relaxivities and their easy uptake by cells.
本研究旨在开发和表征新型表面修饰的氧化铁纳米粒子，以证明其能够被脐血来源的人内皮祖细胞（EPCs）内化的效率。
通过原位沉淀或后合成将聚天冬氨酸-半胱氨酸（PAA-Cys）涂覆在氧化铁纳米粒子上。通过 X 射线粉末衍射对纳米粒子进行表征。用 PAA-Cys 修饰的氧化铁纳米粒子或未涂层的纳米粒子标记 EPCs。测定未涂层和涂层氧化铁纳米粒子以及用 PAA-Cys 修饰的氧化铁标记的 EPCs 的弛豫率。
添加 PAA-Cys 分别将粒径从 10.4nm 增加到 144nm 和 197nm。X 射线粉末衍射图谱表明，这些颗粒由具有螺旋结构的 Fe(3)O(4)组成。后合成涂层颗粒的细胞摄取率分别为 85%和 15.26pg 铁/细胞。对于这两种类型的颗粒，弛豫率比金标准 Resovist(®)至少高 2 倍。
由于 PAA-Cys 涂层氧化铁纳米粒子具有高弛豫率和易于被细胞摄取的特点，因此对于标记活细胞（如干细胞），用于细胞治疗中的诊断和治疗应用具有广阔的应用前景。
