University Clinic of Tübingen, Department of Internal Medicine II, Division of Haematology, Immunology, Oncology, Rheumatology, and Pulmonology, Laboratory for Stem Cell Research, Otfried-Müller-Str. 10, 72076 Tübingen, Germany.
Best Pract Res Clin Haematol. 2011 Mar;24(1):25-36. doi: 10.1016/j.beha.2011.01.001. Epub 2011 Feb 23.
Conventionally, mesenchymal/stromal stem cells (MSC) are functionally isolated from primary tissue based on their capacity to adhere to the plastic surface. This isolation procedure is hampered by the unpredictable influence of secreted molecules or interactions with co-cultured hematopoietic and other unrelated cells as well as by the arbitrarily selected removal time of non-adherent cells prior to expansion of MSC. Early removal of non-adherent cells may result in the elimination of a late adhering MSC subsets and late removal increases the influence of undesired cells on the growth and differentiation of MSC. Finally, in conventional protocols MSC are co-expanded together with macrophages, endothelial cells and other adherent cells. To circumvent these limitations, several strategies have been developed to facilitate the prospective isolation of MSC based on the selective expression or absence of surface markers. Here we summarize the most frequently used markers and introduce new targets for antibody-based isolation procedures of primary bone marrow-derived MSC.
传统上，基于黏附塑料表面的能力，间充质/基质干细胞（MSC）从原组织中分离出来。这种分离过程受到分泌分子的不可预测的影响或与共培养的造血细胞和其他无关细胞的相互作用的阻碍，还受到在 MSC 扩增之前任意选择去除非黏附细胞的时间的阻碍。非黏附细胞的早期去除可能导致晚期黏附 MSC 亚群的消除，而晚期去除增加了不想要的细胞对 MSC 生长和分化的影响。最后，在传统方案中，MSC 与巨噬细胞、内皮细胞和其他黏附细胞共同扩增。为了规避这些限制，已经开发了几种策略，以基于表面标志物的选择性表达或缺失来促进 MSC 的预期分离。在这里，我们总结了最常用的标记物，并为基于抗体的原代骨髓源性 MSC 分离程序引入了新的靶标。
