Department of Chemistry, Indiana University, 800 E. Kirkwood Avenue, Bloomington, Indiana 47405, United States.
ACS Nano. 2011 May 24;5(5):4037-45. doi: 10.1021/nn200629g. Epub 2011 Apr 5.
This article demonstrates the encapsulation of cubic iron oxide nanoparticles (NPs) by Brome mosaic virus capsid shells and the formation, for the first time, of virus-based nanoparticles (VNPs) with cubic cores. Cubic iron oxide NPs functionalized with phospholipids containing poly(ethylene glycol) tails and terminal carboxyl groups exhibited exceptional relaxivity in magnetic resonance imaging experiments, which opens the way for in vivo MRI studies of systemic virus movement in plants. Preliminary data on cell-to-cell and long-distance transit behavior of cubic iron oxide NPs and VNPs in Nicotiana benthamiana leaves indicate that VNPs have specific transit properties, i.e., penetration into tissue and long-distance transfer through the vasculature in N. benthamiana plants, even at low temperature (6 °C), while NPs devoid of virus protein coats exhibit limited transport by comparison. These particles potentially open new opportunities for high-contrast functional imaging in plants and for the delivery of therapeutic antimicrobial cores into plants.
本文展示了立方氧化亚铁纳米粒子（NPs）被 Bromo mosaic 病毒衣壳包裹，并首次形成具有立方核的基于病毒的纳米粒子（VNPs）。用含有聚乙二醇（PEG）尾巴和末端羧基的磷脂功能化的立方氧化亚铁 NPs 在磁共振成像实验中表现出优异的弛豫率，这为在植物体内进行系统病毒运动的 MRI 研究开辟了道路。关于立方氧化亚铁 NPs 和 VNPs 在 Nicotiana benthamiana 叶片中的细胞间和长距离转运行为的初步数据表明，VNPs 具有特定的转运特性，即穿透组织并通过 Nicotiana benthamiana 植物的脉管系统进行长距离转移，即使在低温（6°C）下也是如此，而没有病毒蛋白外壳的 NPs 相比则表现出有限的转运。这些颗粒有可能为植物中的高对比度功能成像以及将治疗性抗菌核心递送到植物中开辟新的机会。
