Department of Blood Transfusion, Xinqiao Hospital, Third Military Medical University, Shapingba District, Chongqing, China.
PLoS One. 2011 Mar 31;6(3):e18328. doi: 10.1371/journal.pone.0018328.
Red blood cell (RBC) storage lesions have been shown to be associated with some adverse reactions; numerous studies have focused on the lesions caused by storage, and few data on the RBC storage lesions caused by prestorage treatments of leucocyte filtration and irradiation. In this study, we examined the changes related with the RBC storage lesions, including 2,3-diphosphatidylglyceric acid (2,3-DPG), pH, free hemoglobin (Hb), supernatant free K+ and Na+ concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH). Along with the increasing storage time, decreases in 2, 3-DPG levels, pH and Na+ concentration, increases in K+ and free Hb concentrations, and significant morphological changes in RBC in all groups were found. The changes in the groups of irradiation, leucocyte filtration and the combined irradiation and leucocyte filtration were more significant than those in the untreated group. Meanwhile, the MCV levels of the three treated groups were significantly lower than those in the untreated group, while the MCH variations were significantly higher. Our results suggest that irradiation and leucocyte filtration before storage may aggravate blood storage lesions.
红细胞（RBC）储存损伤与一些不良反应有关；许多研究都集中在储存引起的损伤上，而关于白细胞过滤和辐照预处理引起的 RBC 储存损伤的数据较少。在这项研究中，我们检查了与 RBC 储存损伤相关的变化，包括 2,3-二磷酸甘油酸（2,3-DPG）、pH 值、游离血红蛋白（Hb）、上清液游离 K+和 Na+浓度、平均红细胞体积（MCV）、平均红细胞血红蛋白（MCH）。随着储存时间的延长，我们发现 2,3-DPG 水平、pH 值和 Na+浓度降低，K+和游离 Hb 浓度升高，所有组的 RBC 形态发生显著变化。辐照组、白细胞过滤组和联合辐照及白细胞过滤组的变化比未处理组更为显著。同时，三个处理组的 MCV 水平明显低于未处理组，而 MCH 的变化明显更高。我们的结果表明，储存前的辐照和白细胞过滤可能会加重血液储存损伤。
