The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Science. 2011 Apr 15;332(6027):342-6. doi: 10.1126/science.1202998.
We present a strategy to design and construct self-assembling DNA nanostructures that define intricate curved surfaces in three-dimensional (3D) space using the DNA origami folding technique. Double-helical DNA is bent to follow the rounded contours of the target object, and potential strand crossovers are subsequently identified. Concentric rings of DNA are used to generate in-plane curvature, constrained to 2D by rationally designed geometries and crossover networks. Out-of-plane curvature is introduced by adjusting the particular position and pattern of crossovers between adjacent DNA double helices, whose conformation often deviates from the natural, B-form twist density. A series of DNA nanostructures with high curvature--such as 2D arrangements of concentric rings and 3D spherical shells, ellipsoidal shells, and a nanoflask--were assembled.
我们提出了一种设计和构建自组装 DNA 纳米结构的策略，该策略使用 DNA 折纸折叠技术在三维（3D）空间中定义复杂的曲面。双链 DNA 被弯曲以跟随目标物体的圆形轮廓，随后确定潜在的链交叉。同心 DNA 环用于产生平面曲率，通过合理设计的几何形状和交叉网络约束在 2D 上。通过调整相邻 DNA 双螺旋之间交叉的特定位置和模式引入面外曲率，其构象通常偏离自然的 B 型扭曲密度。一系列具有高曲率的 DNA 纳米结构，例如同心环的 2D 排列以及 3D 球形壳、椭球形壳和纳米瓶，被组装。
