Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Biomacromolecules. 2012 Feb 13;13(2):517-27. doi: 10.1021/bm2015834. Epub 2012 Jan 30.
Dextrin, a glucose polymer with low molecular weight, was used to develop a fully resorbable hydrogel, without using chemical initiators. Dextrin was first oxidized (oDex) with sodium periodate and then cross-linked with adipic acid dihidrazide, a nontoxic cross-linking molecule. Furthermore, a new bidimensional composite hydrogel, made of oxidized dextrin incorporating dextrin nanogels (oDex-nanogel), was also developed. The oDex hydrogels showed good mechanical properties and biocompatibility, allowing the proliferation of mouse embryo fibroblasts 3T3 cultured on top of the gel. The gelation time may be controlled selecting the concentrations of the polymer and reticulating agent. Both the oDex and oDex-nanogel hydrogels are biodegradable and present a 3-D network with a continuous porous structure. The obtained hybrid hydrogel enables the release of the dextrin nanogel over an extended period of time, paralleling the mass loss curve due to the degradation of the material. The dextrin nanogel allowed the efficient incorporation of interleukin-10 and insulin in the oDex hydrogel, providing a sophisticated system of controlled release. The new hydrogels present promising properties as an injectable carrier of bioactive molecules. Both proteins and poorly water-soluble low-molecular-weight drugs are efficiently encapsulated in the nanogel, which performs as a controlled release system entrapped in the hydrogel matrix.
糊精是一种低分子量的葡萄糖聚合物，被用来开发一种完全可吸收的水凝胶，而无需使用化学引发剂。糊精首先用高碘酸钠氧化（oDex），然后用非毒性交联分子己二酸二酰肼交联。此外，还开发了一种新的二维复合水凝胶，由氧化糊精和糊精纳米凝胶（oDex-纳米凝胶）组成。oDex 水凝胶具有良好的机械性能和生物相容性，允许在凝胶顶部培养的小鼠胚胎成纤维细胞 3T3 增殖。凝胶时间可以通过选择聚合物和交联剂的浓度来控制。oDex 和 oDex-纳米凝胶水凝胶都是可生物降解的，并且具有连续多孔结构的 3-D 网络。所得到的杂化水凝胶能够在较长时间内释放糊精纳米凝胶，与由于材料降解而导致的质量损失曲线平行。糊精纳米凝胶允许将白细胞介素 10 和胰岛素有效地掺入 oDex 水凝胶中，提供了一种复杂的控制释放系统。新的水凝胶具有作为生物活性分子的可注射载体的有前途的特性。蛋白质和水溶性差的低分子量药物都可以有效地包封在纳米凝胶中，纳米凝胶作为被包埋在水凝胶基质中的控制释放系统发挥作用。
