伊朗东南部患者白细胞介素-4基因多态性与多发性硬化症的关联
Association of interleukin-4 polymorphisms with multiple sclerosis in southeastern Iranian patients.
作者信息
Arababadi Mohammad Kazemi, Mosavi Reza, Ravari Ali, Teimori Hossein, Hassanshahi Gholamhossein
机构信息
Department of Microbiology, Hematology and Immunology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Iran.
出版信息
Ann Saudi Med. 2012 Mar-Apr;32(2):127-30. doi: 10.5144/0256-4947.2012.127.
BACKGROUND AND OBJECTIVES
Immune system-related factors are important in the pathogenesis of multiple sclerosis (MS). Interleukin 4 (IL-4) as a helper T cell (2TH) cytokine is involved in the regulation of immune responses. Hence, this study was designed to explore the association between MS and polymorphisms in the -590 region of IL-4.
DESIGN AND SETTING
A descriptive study at Rafsanjan University of Medical Sciences, Rafsnajan from September 2009 to August 2010.
PATIENTS AND METHODS
Blood samples were collected from 100 MS patients and 150 healthy controls on EDTA precoated tubes. DNA was extracted and analyzed for IL-4 polymorphisms using restricted fragment length polymorphism in patients and controls. Demographic data were also collected by a questionnaire that was designed specifically for this study.
RESULTS
We observed a significant difference in the C/C, T/C, and T/T genotypes of the -590 region of IL-4 between patients with MS and healthy controls (P<.001).
CONCLUSIONS
We conclude that functional polymorphisms of IL-4 possibly play a crucial role in the pathogenesis of MS.
背景与目的
免疫系统相关因素在多发性硬化症（MS）的发病机制中起重要作用。白细胞介素4（IL-4）作为辅助性T细胞（2TH）细胞因子参与免疫反应的调节。因此，本研究旨在探讨MS与IL-4基因-590区域多态性之间的关联。
设计与地点
2009年9月至2010年8月在拉夫桑詹医科大学进行的一项描述性研究。
患者与方法
用乙二胺四乙酸（EDTA）预包被管从100例MS患者和150例健康对照者采集血样。提取DNA，采用限制性片段长度多态性分析患者和对照者的IL-4基因多态性。还通过专门为本研究设计的问卷收集人口统计学数据。
结果
我们观察到MS患者与健康对照者在IL-4基因-590区域的C/C、T/C和T/T基因型存在显著差异（P<0.001）。
结论
我们得出结论，IL-4的功能性多态性可能在MS的发病机制中起关键作用。
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