吡拉西坦和长春西汀可改善鱼藤酮诱导的大鼠帕金森病。

Piracetam and vinpocetine ameliorate rotenone-induced Parkinsonism in rats.

机构信息

Department of Pharmacology and Toxicology, Suez Canal University, Ismailia, Egypt.

出版信息

Indian J Pharmacol. 2012 Nov-Dec;44(6):774-9. doi: 10.4103/0253-7613.103300.

DOI:10.4103/0253-7613.103300

PMID:23248410

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3523508/

Abstract

OBJECTIVE

To evaluate the neuroprotective effect of the nootropic drugs, piracetam (PIR) and vinpocetine (VIN), in rotenone-induced Parkinsonism in rats.

MATERIALS AND METHODS

Sixty male rats were divided into 6 groups of 10 rats each. The groups were administered vehicle, control (rotenone, 1.5 mg/kg/48 h/6 doses, s.c.), PIR (100 and 200 mg/kg/day, p.o.) and VIN (3 and 6 mg/kg/day, p.o.). The motor performance of the rats was evaluated by the open field and pole test. Striatal dopamine level, malondialdehyde (MDA), reduced glutathione (GSH) and tumor necrosis factor-α (TNF-α) were assayed. Histopathological study of the substantia nigra was also done.

RESULTS

Results showed that rotenone-treated rats exhibited bradykinesia and motor impairment in the open-field test. In addition, GSH level was decreased whereas MDA and TNF-α increased in striata of rotenone-treated rats as compared to vehicle-treated rats. Marked degeneration of the substantia nigra pars compacta (SNpc) neurons and depletion of striatal dopamine was also observed in the rotenone-treated rats. Treatment with PIR or VIN significantly reversed the locomotor deficits and increased striatal dopamine level. Treatment with VIN significantly (P<0.05) reduced the striatal level of MDA and GSH in comparison to rotenone group whereas TNF-α production was found to be significantly decreased in PIR group (P<0.05).

CONCLUSION

VIN and PIR exhibit neuroprotective activity in rotenone-induced Parkinsonism. Hence, these nootropic agents may be considered as possible candidates in the treatment of Parkinson's disease.

摘要

目的

评估益智药吡拉西坦（PIR）和长春西汀（VIN）对鱼藤酮诱导的大鼠帕金森病的神经保护作用。

材料与方法

将 60 只雄性大鼠分为 6 组，每组 10 只。各组给予载体、对照（鱼藤酮，1.5mg/kg/48h/6 次，sc）、PIR（100 和 200mg/kg/天，po）和 VIN（3 和 6mg/kg/天，po）。通过旷场和棒试验评估大鼠的运动性能。测定纹状体多巴胺水平、丙二醛（MDA）、还原型谷胱甘肽（GSH）和肿瘤坏死因子-α（TNF-α）。还对黑质进行了组织病理学研究。

结果

结果表明，鱼藤酮处理的大鼠在旷场试验中表现出运动迟缓。此外，与载体处理的大鼠相比，鱼藤酮处理的大鼠纹状体中的 GSH 水平降低，MDA 和 TNF-α 水平升高。在鱼藤酮处理的大鼠中还观察到黑质致密部（SNpc）神经元明显退化和纹状体多巴胺耗竭。PIR 或 VIN 治疗显著逆转了运动缺陷并增加了纹状体多巴胺水平。与鱼藤酮组相比，VIN 治疗显著（P<0.05）降低了纹状体 MDA 水平和 GSH 水平，而 TNF-α 产生在 PIR 组中发现明显降低（P<0.05）。