Yang Lin, You Sixian, Zhang Liangkai, Yang Tixiong, Li Pengcheng, Lu Jinling
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China ; These authors contributed equally.
Biomed Opt Express. 2013 Jan 1;4(1):32-7. doi: 10.1364/BOE.4.000032. Epub 2012 Dec 10.
Monitoring the vital signs of a developing embryo is very useful in avian breeding programs, especially during early days of incubation, so that dead or unfertilized eggs can be timely removed from incubator and new eggs can be placed in. A noninvasive system for detecting the vital signs of avian embryo through intact egg in early stage of incubation has been developed using laser speckle imaging (LSI). The system was based on the measurement of intensity fluctuations of speckle caused by the embryo's blood flow in the intact egg under laser light illumination. This system was found to be feasible in imaging the vasculature in the egg as well as confirming its fertilization or survival from the second day to fifth day of incubation while other reported noninvasive methods cannot detect vital signs of the embryo until the sixth day of incubation.
监测发育中胚胎的生命体征在禽类育种计划中非常有用，尤其是在孵化初期，这样可以及时从孵化器中取出死蛋或未受精的蛋，并放入新的蛋。利用激光散斑成像（LSI）开发了一种用于在孵化早期通过完整鸡蛋检测禽类胚胎生命体征的非侵入性系统。该系统基于测量激光照射下完整鸡蛋中胚胎血流引起的散斑强度波动。结果发现，该系统在对鸡蛋中的血管系统进行成像以及确认其在孵化第二天至第五天的受精或存活情况方面是可行的，而其他已报道的非侵入性方法直到孵化第六天才能够检测到胚胎的生命体征。
