PR3-ANCA：广泛疾病溃疡性结肠炎有前景的生物标志物。
PR3-ANCA: a promising biomarker for ulcerative colitis with extensive disease.
机构信息
INOVA Diagnostics, Inc., San Diego, CA, United States.
出版信息
Clin Chim Acta. 2013 Sep 23;424:267-73. doi: 10.1016/j.cca.2013.06.005. Epub 2013 Jun 24.
BACKGROUND
We determined if PR3-ANCA is a biomarker that differentiates ulcerative colitis (UC) from Crohn's disease (CrD).
METHODS
A total of 946 sera were tested, including 86 granulomatosis with polyangiitis (GPA) and 491 inflammatory bowel disease (IBD) patients (283 UC and 208 CrD), 264 pathological controls (various diseases) and 105 healthy individuals. All samples were tested for PR3-ANCA by ELISA (QUANTA Flash Lite®, INOVA Diagnostics) and chemiluminescent immunoassays (CIA QUANTA Flash PR3). Conventional anti-neutrophil cytoplasmic antibody (ANCA) indirect immunofluorescence assays (IIF) was performed with NOVA Lite™ (INOVA Diagnostics).
RESULTS
PR3-ANCA by CIA were detected in 31.1% UC vs. 1.9% CrD sera (p=2.2E-16), and by ELISA in 6% UC and 0% CrD (p=0.0003). In GPA patients, PR3-ANCA were detected in 75.6% by CIA and 61.6% by ELISA (p<0.05). PR3-ANCA by CIA were more prevalent in E3-UC compared to E1/2-UC (p<0.05), and in patients with shorter disease duration (p<0.0001). PR3-ANCA showed similar sensitivity, but significantly higher specificity (p<0.05), compared to atypical pANCA by IIF.
CONCLUSION
The novel PR3 CIA may prove helpful in the differentiation of CrD from UC, as well as in the identification of UC patients with more extensive disease.
背景
我们旨在确定蛋白酶 3-抗中性粒细胞胞质抗体（PR3-ANCA）是否为鉴别溃疡性结肠炎（UC）与克罗恩病（CrD）的生物标志物。
方法
共检测了 946 份血清，包括 86 例肉芽肿性多血管炎（GPA）和 491 例炎症性肠病（IBD）患者（283 例 UC 和 208 例 CrD）、264 例病理对照（各种疾病）和 105 例健康个体。所有样本均通过 ELISA（QUANTA Flash Lite®，INOVA Diagnostics）和化学发光免疫分析（CIA QUANTA Flash PR3）检测 PR3-ANCA。采用 NOVA Lite™（INOVA Diagnostics）进行常规抗中性粒细胞胞质抗体（ANCA）间接免疫荧光检测（IIF）。
结果
CIA 检测到 PR3-ANCA 在 31.1%的 UC 血清中，而在 1.9%的 CrD 血清中（p=2.2E-16），ELISA 检测到 6%的 UC 和 0%的 CrD（p=0.0003）。在 GPA 患者中，CIA 检测到 75.6%的 PR3-ANCA，ELISA 检测到 61.6%的 PR3-ANCA（p<0.05）。CIA 检测到的 PR3-ANCA 在 E3-UC 中比 E1/2-UC 更常见（p<0.05），且在疾病病程较短的患者中更常见（p<0.0001）。与 IIF 检测到的非典型 pANCA 相比，PR3-ANCA 具有相似的敏感性，但特异性更高（p<0.05）。
结论
新型 PR3 CIA 可能有助于鉴别 CrD 与 UC，以及识别疾病范围更广的 UC 患者。
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