Department of Orthopaedics, The First Affiliated Hospital, Liaoning Medical University, People Street 2-5, GuTa District, Jinzhou, 121001, Liaoning Province, China.
J Mol Neurosci. 2013 Nov;51(3):986-93. doi: 10.1007/s12031-013-0083-8. Epub 2013 Aug 14.
Salvianolic acid B (Sal B), a bioactive compound isolated from the Chinese medicinal herb danshen, is commonly used for the prevention and treatment of cardiovascular disease. The present study was performed to investigate the effect of Sal B on the blood-spinal cord barrier (BSCB) after spinal cord injury (SCI) in a rat model. Sal B (1, 10, and 50 mg/kg i.v.) was administered to rats immediately following SCI. The permeability of the BSCB and spinal cord tissue water content were evaluated. Additionally, the expression levels of tight junction proteins and heme oxygenase-1 (HO-1) were monitored by Western blot analysis. Enzyme-linked immunosorbent assay analysis of spinal cord tissue homogenates was performed 24 h post-SCI to evaluate the expression of inflammation-related cytokines. In addition, the motor recovery of SCI rats was assessed using the Basso, Beattie, and Bresnahan scoring system. Compared to the SCI group, rats treated with Sal B (10, 50 mg/kg) exhibited significantly reduced spinal cord tissue water content and BSCB permeability. Further, the motor function of rats was also greatly improved by Sal B administration. The expression of pro-inflammatory factors TNF-α and NF-κB was found to be greatly increased 24 h post-SCI, and this upregulation was significantly attenuated by Sal B treatment. The expression of ZO-1 and occludin was upregulated by Sal B (10 mg/kg) treatment after SCI, and this effect was blocked by the HO-1 inhibitor ZnPP. Taken together, our results clearly indicate that Sal B attenuates SCI by promoting the repair of the damaged BSCB, demonstrating that this molecule is a novel and promising therapeutic agent for human SCI.
丹酚酸 B（Sal B）是从中药丹参中分离得到的一种生物活性化合物，常用于预防和治疗心血管疾病。本研究旨在探讨丹酚酸 B 对大鼠脊髓损伤（SCI）后血脊髓屏障（BSCB）的影响。SCI 后立即给予 Sal B（1、10 和 50mg/kg，iv）。评估 BSCB 的通透性和脊髓组织含水量。此外，通过 Western blot 分析监测紧密连接蛋白和血红素加氧酶-1（HO-1）的表达水平。SCI 后 24h 通过酶联免疫吸附试验分析脊髓组织匀浆，评估炎症相关细胞因子的表达。此外，使用 Basso、Beattie 和 Bresnahan 评分系统评估 SCI 大鼠的运动功能恢复情况。与 SCI 组相比，Sal B（10、50mg/kg）治疗的大鼠脊髓组织水含量和 BSCB 通透性明显降低。此外，Sal B 给药还极大地改善了大鼠的运动功能。SCI 后 24h，促炎因子 TNF-α和 NF-κB 的表达明显增加，Sal B 治疗显著减弱了这种上调。SCI 后 Sal B（10mg/kg）治疗可上调 ZO-1 和 occludin 的表达，而这种作用被 HO-1 抑制剂 ZnPP 阻断。综上所述，我们的研究结果清楚地表明，Sal B 通过促进受损 BSCB 的修复来减轻 SCI，表明该分子是治疗人类 SCI 的一种新型有前途的治疗剂。
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