构建、测试和验证一组自制的von Frey细丝：一种用于伤害感受评估的精确、准确且经济高效的替代方法。
Building, testing and validating a set of home-made von Frey filaments: a precise, accurate and cost effective alternative for nociception assessment.
作者信息
de Sousa Marcelo Victor Pires, Ferraresi Cleber, de Magalhães Ana Carolina, Yoshimura Elisabeth Mateus, Hamblin Michael R
机构信息
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA; Laboratory of Radiation Dosimetry and Medical Physics, Institute of Physics, São Paulo University, São Paulo, Brazil.
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA; Laboratory of Electro-thermo-phototherapy, Department of Physical Therapy, Federal University of São Carlos, São Paulo, Brazil; Post-Graduation Program in Biotechnology, Federal University of São Carlos, São Paulo, Brazil; Optics Group, Physics Institute of Sao Carlos, University of São Paulo, São Carlos, Brazil.
出版信息
J Neurosci Methods. 2014 Jul 30;232:1-5. doi: 10.1016/j.jneumeth.2014.04.017. Epub 2014 May 2.
BACKGROUND
A von Frey filament (vFF) is a type of aesthesiometer usually made of nylon perpendicularly held in a base. It can be used in paw withdrawal pain threshold assessment, one of the most popular tests for pain evaluation using animal models. For this test, a set of filaments, each able to exert a different force, is applied to the animal paw, from the weakest to the strongest, until the paw is withdrawn.
NEW METHOD
We made 20 low cost vFF using nylon filaments of different lengths and constant diameter glued perpendicularly to the ends of popsicle sticks. They were calibrated using a laboratory balance scale. Building and calibrating took around 4h and confirmed the theoretical prediction that the force exerted is inversely proportional to the length and directly proportional to the width of the filament.
RESULTS
The calibration showed that they were precise and accurate. We analyzed the paw withdrawal threshold assessed with the set of home-made vFF and with a high quality commercial set of 5 monofilaments vFF (Stoelting, Wood Dale, USA) in two groups (n=5) of healthy mice.
COMPARISON WITH EXISTING METHODS
The home-made vFF precisely and accurately measured the hind paw withdrawal threshold (20.3±0.9 g). The commercial vFF have different diameters while our set has the same diameter avoiding the problem of lower sensitivity to larger diameter filaments.
CONCLUSION
Building a set of vFF is easy, cost effective, and depending on the kind of tests, can increase precision and accuracy of animal nociception evaluation.
背景
von Frey细丝（vFF）是一种通常由垂直固定在基座中的尼龙制成的触觉测量仪。它可用于爪缩足疼痛阈值评估，这是使用动物模型进行疼痛评估最常用的测试之一。在该测试中，将一组能施加不同力的细丝从最弱到最强依次施加到动物爪子上，直到爪子缩回。
新方法
我们使用不同长度、直径恒定的尼龙细丝垂直粘贴在冰棒棍末端，制作了20个低成本的vFF。它们使用实验室天平进行校准。制作和校准耗时约4小时，并证实了理论预测，即施加的力与细丝长度成反比，与细丝宽度成正比。
结果
校准显示它们精确且准确。我们在两组（每组n = 5）健康小鼠中，分析了用自制vFF组和一套高质量的5根单丝商业vFF（美国伊利诺伊州绍尔廷市的Stoelting公司生产）评估的爪缩足阈值。
与现有方法的比较
自制vFF精确且准确地测量了后爪缩足阈值（20.3±0.9克）。商业vFF有不同直径，而我们的套件直径相同，避免了对较大直径细丝敏感性较低的问题。
结论
制作一套vFF简单、经济高效，并且根据测试类型，可提高动物伤害感受评估的精度和准确性。
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