Laboratory of Cytophysiology, Institute of Marine Biology, Far East Branch, Russian Academy of Sciences, Vladivostok 690059, Russia.
St. Petersburg State University, St. Petersburg 199034, Russia.
Neural Regen Res. 2013 Jan 5;8(1):13-23. doi: 10.3969/j.issn.1673-5374.2013.01.002.
We investigated the distribution of gamma aminobutyric acid, tyrosine hydroxylase and nitric oxide-producing elements in a cherry salmon Oncorhynchus masou brain at various stages of postnatal ontogenesis by immunohistochemical staining and histochemical staining. The periventricular region cells exhibited the morphology of neurons and glia including radial glia-like cells and contained several neurochemical substances. Heterogeneous populations of tyrosine hydroxylase-, gamma aminobutyric acid-immunoreactive, as well as nicotinamide adenine dinucleotide phosphate diaphorase-positive cells were observed in proliferating cell nuclear antigen-immunoreactive proliferative zones in periventricular area of diencephalon, central grey layer of dorsomedial tegmentum, medulla and spinal cord. Immunolocalization of Pax6 in the cherry salmon brain revealed a neuromeric construction of the brain at various stages of postnatal ontogenesis, and this was confirmed by tyrosine hydroxylase and gamma aminobutyric acid labeling.
我们通过免疫组织化学染色和组织化学染色研究了在不同出生后发育阶段的虹鳟鱼 Oncorhynchus masou 大脑中γ-氨基丁酸、酪氨酸羟化酶和产生一氧化氮的元素的分布。室周区细胞表现出神经元和神经胶质的形态，包括放射状胶质样细胞，并含有几种神经化学物质。在间脑室周区、背内侧中脑被盖的中央灰色层、延髓和脊髓的增殖细胞核抗原免疫反应性增殖区中，观察到酪氨酸羟化酶、γ-氨基丁酸免疫反应性以及烟酰胺腺嘌呤二核苷酸磷酸二氢酶阳性细胞的异质群体。在虹鳟鱼脑中的 Pax6 免疫定位显示了在出生后发育的不同阶段的脑的神经节结构，并且这通过酪氨酸羟化酶和γ-氨基丁酸标记得到了证实。
