一种用于确定腈水解酶底物范围的高通量筛选方法。
A high-throughput screening method for determining the substrate scope of nitrilases.
作者信息
Black Gary W, Brown Nicola L, Perry Justin J B, Randall P David, Turnbull Graeme, Zhang Meng
机构信息
Industrial Biotechnology Research Group, Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK.
出版信息
Chem Commun (Camb). 2015 Feb 14;51(13):2660-2. doi: 10.1039/c4cc06021k.
Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.
腈类化合物是合成诸如阿托伐他汀等药物的中间体。我们开发了一种显色试剂来筛选腈水解酶活性，以替代奈斯勒试剂。它会产生一种半定量的蓝色，并且已经证明23种腈水解酶作为无细胞提取物可水解38种腈底物。
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