Lobo Sonja E, Glickman Robert, da Silva Wagner N, Arinzeh Treena L, Kerkis Irina
Department of Morphology and Genetics, Federal University of São Paulo, São Paulo, Brazil,
Cell Tissue Res. 2015 Aug;361(2):477-95. doi: 10.1007/s00441-015-2116-9. Epub 2015 Feb 13.
Biphasic calcium phosphate (BCP) bioceramics have been successfully applied in a broad variety of presentation forms and with different ratios of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP). BCPs have been loaded with stem cells from different origins for bone tissue engineering purposes, but evidence of stem cell behavior on different compositions (various HA/β-TCP ratios) and physical features of BCPs is limited. We compared the adhesion, proliferation, viability and osteogenic potential of human mesenchymal stem cells (MSCs) on granular BCPs with equal HA/β-TCP ratio of diverse particle sizes and on porous blocks which had different chemical compositions. In addition, the osteogenic differentiation of MSCs was compared to adipose-derived (ADSC) and dental pulp (DPSC) stem cells, as well as to pre-osteoblasts on a particulate BCP. MSCs growing on granular BCPs demonstrated increased number as compared to MSCs growing on blocks. Cells proliferated to a greater extent on small granular BCPs, while large granular BCPs and blocks promoted cell differentiation. Surprisingly, the expression of genes involved in osteogenesis was upregulated in MSCs on bioceramics in basal medium which indicates that BCPs may have osteoinductive potential. This was confirmed with the upregulation of osteochondrogenic markers, at different time points, when stem cells from various tissues were grown on the BCP. This study demonstrates that BCPs, depending on their physical features and chemical composition, modulate stem cell behavior, and that stem cells from different origins are inherently distinct in their gene expression profile and can be triggered toward osteochondrogenic fate by BCPs.
双相磷酸钙（BCP）生物陶瓷已成功应用于多种呈现形式，且具有不同比例的羟基磷灰石（HA）和β-磷酸三钙（β-TCP）。为了骨组织工程目的，BCP已负载来自不同来源的干细胞，但关于干细胞在不同组成（各种HA/β-TCP比例）和物理特征的BCP上行为的证据有限。我们比较了人骨髓间充质干细胞（MSCs）在具有相同HA/β-TCP比例但颗粒大小不同的颗粒状BCP上以及在具有不同化学成分的多孔块上的黏附、增殖、活力和成骨潜力。此外，还将MSCs的成骨分化与脂肪来源（ADSC）和牙髓（DPSC）干细胞以及颗粒状BCP上的前成骨细胞进行了比较。与在块状BCP上生长的MSCs相比，在颗粒状BCP上生长的MSCs数量增加。细胞在小颗粒状BCP上增殖程度更大，而大颗粒状BCP和块状BCP促进细胞分化。令人惊讶的是，基础培养基中生物陶瓷上的MSCs中参与成骨的基因表达上调，这表明BCP可能具有骨诱导潜力。当来自不同组织的干细胞在BCP上生长时，在不同时间点骨软骨生成标志物的上调证实了这一点。这项研究表明，BCP根据其物理特征和化学成分调节干细胞行为，并且来自不同来源的干细胞在基因表达谱上本质上是不同的，并且可以被BCP触发走向骨软骨生成命运。
