评估明矾-纳曲酮佐剂活性对用高压灭菌的硕大利什曼原虫（MRHO/IR/75/ER）抗原免疫BALB/C小鼠抗利什曼原虫效果的影响。

Evaluation of Alum-Naltrexone Adjuvant Activity, on Efficacy of Anti-Leishmania Immunization with Autoclaved Leishmania major (MRHO/IR/75/ER) Antigens in BALB/C Mice.

作者信息

Mohammadzadeh Hajipirloo Habib, Bozorgomd Arezoo, Shahabi Shahram, Hazrati Tappeh Khosrow, Karamati Seyed Ahmad

机构信息

Dept. of Medical Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.

Dept. of Microbiology, Immunology and Genetics, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.

出版信息

Iran J Parasitol. 2014 Sep;9(3):311-8.

DOI:

PMID:25678914

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4316561/

Abstract

BACKGROUND

Naltrexone, an opioid receptor antagonist shifts the immune response toward a Th1 profile. In the current study, we evaluated the efficacy of the mixture of NTX and alum, as a new adjuvant, to enhance immune response and induce protection against Leishmania major in a mouse model.

METHODS

BALB/c mice were immunized three times either autoclaved L. major promastigotes' antigens alone or in combination with the adjuvant alum, naltrexone or the alum-naltrexone mixture. Both humoral and cellular immune responses were assessed two weeks after the last immunization and compared with control mice.

RESULTS

The administration of alum- NTX in combination with the parasite antigen, significantly increased production of IFN-γ IFN-γ /IL-5 ratio, lymphocyte proliferation and improved DTH response against L. major. There was no significant difference in survival following challenge among groups.

CONCLUSION

Immunization with the alum- naltrexone mixture as an adjuvant, in combination with the autoclaved L. major promastigotes antigens, can enhance cellular immunity and shift the immune responses to a Th1 pattern.

摘要