Kuroda Mondo, Shimizu Masaki, Inoue Natsumi, Ikeno Iku, Nakagawa Hiroyasu, Yokoi Ayano, Niida Yo, Konishi Michio, Kaneda Hisashi, Igarashi Noboru, Yamahana Junya, Taneichi Hiromichi, Kanegane Hirokazu, Ito Mika, Saito Shigeru, Furuichi Kengo, Wada Takashi, Nakagawa Masaru, Yokoyama Hitoshi, Yachie Akihiro
Department of Pediatrics, School of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
Department of Pediatrics, School of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
Neurochem Int. 2015 Jun-Jul;85-86:24-30. doi: 10.1016/j.neuint.2015.04.003. Epub 2015 Apr 17.
Tau protein levels in cerebrospinal fluid (CSF) and serum are elevated in patients with various central nervous system diseases. We investigated whether serum tau protein levels are useful for predicting and assessing disease activity of acute encephalopathy (AE) in enterohemorrhagic Escherichia coli (EHEC) O111-induced hemolytic uremic syndrome (HUS; EHEC encephalopathy). Serum samples were obtained from 14 patients with EHEC O111/HUS, 20 patients with non-EHEC-related AE, and 20 age- and sex-matched healthy controls. CSF samples were obtained from 2 patients with EHEC encephalopathy and 20 patients with non-EHEC-related AE. Tau protein levels and levels of several proinflammatory cytokines were quantified by enzyme-linked immunosorbent assays. Results were compared with the clinical features of EHEC encephalopathy, including magnetic resonance image (MRI) findings. Serum tau levels in patients with EHEC encephalopathy were significantly elevated compared with those in patients with EHEC O111/HUS without encephalopathy, patients with non-EHEC-related AE, and healthy controls. The ratio of CSF tau levels to serum tau levels was >1.0 in all patients with non-EHEC-related AE but <1.0 in 2 patients with EHEC encephalopathy. Serum tau protein levels increased rapidly and markedly in patients with severe EHEC 0111/HUS and encephalopathy when HUS occurred, but were not elevated in mild patients, even in the HUS phase. Furthermore, changes in serum tau protein levels in patients with EHEC encephalopathy were consistent with abnormalities on brain MRI and were positively correlated with proinflammatory cytokine levels. Our results indicate that serum tau protein might be useful to predict and assess disease activity of EHEC encephalopathy.
在患有各种中枢神经系统疾病的患者中，脑脊液（CSF）和血清中的tau蛋白水平会升高。我们研究了血清tau蛋白水平是否有助于预测和评估肠出血性大肠杆菌（EHEC）O111诱导的溶血尿毒综合征（HUS；EHEC脑病）中急性脑病（AE）的疾病活动情况。从14例EHEC O111/HUS患者、20例非EHEC相关AE患者以及20例年龄和性别匹配的健康对照者中采集血清样本。从2例EHEC脑病患者和20例非EHEC相关AE患者中采集脑脊液样本。通过酶联免疫吸附测定法定量tau蛋白水平和几种促炎细胞因子的水平。将结果与EHEC脑病的临床特征（包括磁共振成像（MRI）结果）进行比较。与无脑病的EHEC O111/HUS患者、非EHEC相关AE患者和健康对照者相比，EHEC脑病患者的血清tau水平显著升高。在所有非EHEC相关AE患者中，脑脊液tau水平与血清tau水平的比值>1.0，但在2例EHEC脑病患者中<1.0。在发生HUS时，患有严重EHEC 0111/HUS和脑病的患者血清tau蛋白水平迅速且显著升高，但轻度患者即使在HUS阶段也未升高。此外，EHEC脑病患者血清tau蛋白水平的变化与脑MRI异常一致，且与促炎细胞因子水平呈正相关。我们的结果表明，血清tau蛋白可能有助于预测和评估EHEC脑病的疾病活动情况。
