2型抗性淀粉对马拉维农村儿童微生物群和肠道炎症标志物的影响。

The effect of dietary resistant starch type 2 on the microbiota and markers of gut inflammation in rural Malawi children.

作者信息

Ordiz M Isabel, May Thaddaeus D, Mihindukulasuriya Kathie, Martin John, Crowley Jan, Tarr Phillip I, Ryan Kelsey, Mortimer Elissa, Gopalsamy Geetha, Maleta Ken, Mitreva Makedonka, Young Graeme, Manary Mark J

机构信息

Department of Pediatrics, Washington University, St. Louis, MO, 63110, USA.

Department of Pediatrics, Baylor College of Medicine, Houston, TX, 77030, USA.

出版信息

Microbiome. 2015 Sep 3;3:37. doi: 10.1186/s40168-015-0102-9.

DOI:10.1186/s40168-015-0102-9

PMID:26334878

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4558878/

Abstract

BACKGROUND

Resistant starch (RS) decreases intestinal inflammation in some settings. We tested the hypothesis that gut inflammation will be reduced with dietary supplementation with RS in rural Malawian children. Eighteen stunted 3-5-year-old children were supplemented with 8.5 g/day of RS type 2 for 4 weeks. The fecal samples were analyzed for the microbiota, the microbiome, short chain fatty acids, metabolome, and proteins indicative of inflammation before and after the intervention. Subjects served as their own controls.

RESULTS

The consumption of RS changed the composition of the microbiota; at the phylum level Actinobacteria increased, while Firmicutes decreased. Among the most prevalent genera, Lactobacillus was increased and Roseburia, Blautia, and Lachnospiracea incertae sedis were decreased. The Shannon H index at the genus level decreased from 2.02 on the habitual diet and 1.76 after the introduction of RS (P < 0.01). Fecal acetate concentration decreased, and fecal propionate concentration increased after RS administration (-5.2 and 2.0 μmol/g, respectively). Fecal calprotectin increased from 29 ± 69 to 89 ± 49 μg/g (P = 0.003) after RS was given. The lipopolysaccharide biosynthesis pathway was upregulated.

CONCLUSIONS

Our findings do not support the hypothesis that RS reduces gut inflammation in rural Malawian children.

摘要

背景

抗性淀粉（RS）在某些情况下可减轻肠道炎症。我们检验了在马拉维农村儿童中通过饮食补充RS可减轻肠道炎症这一假设。18名发育迟缓的3至5岁儿童每天补充8.5克2型RS，为期4周。在干预前后对粪便样本进行微生物群、微生物组、短链脂肪酸、代谢组以及指示炎症的蛋白质分析。受试者自身作为对照。

结果

食用RS改变了微生物群的组成；在门水平上，放线菌门增加，而厚壁菌门减少。在最常见的属中，乳酸杆菌属增加，而罗斯氏菌属、布劳特氏菌属和未分类的毛螺菌科减少。属水平的香农H指数从习惯饮食时的2.02降至引入RS后的1.76（P < 0.01）。给予RS后，粪便乙酸盐浓度降低，粪便丙酸盐浓度升高（分别为-5.2和2.0 μmol/g）。给予RS后，粪便钙卫蛋白从29±69微克/克增加至89±49微克/克（P = 0.003）。脂多糖生物合成途径上调。