Liu F, Weir B S, Damm U, Crous P W, Wang Y, Liu B, Wang M, Zhang M, Cai L
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China;; Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Landcare Research, Private Bag 92170 Auckland, New Zealand.
Persoonia. 2015 Dec;35:63-86. doi: 10.3767/003158515X687597. Epub 2015 Feb 18.
We investigated the phylogenetic diversity of 144 Colletotrichum isolates associated with symptomatic and asymptomatic tissues of Camellia sinensis and other Camellia spp. from seven provinces in China (Fujian, Guizhou, Henan, Jiangxi, Sichuan, Yunnan, Zhejiang), and seven isolates obtained from other countries, including Indonesia, UK, and the USA. Based on multi-locus (ACT, ApMat, CAL, GAPDH, GS, ITS, TUB2) phylogenetic analyses and phenotypic characters, 11 species were distinguished, including nine well-characterised species (C. alienum, C. boninense, C. camelliae, C. cliviae, C. fioriniae, C. fructicola, C. gloeosporioides, C. karstii, C. sia-mense), and two novel species (C. henanense and C. jiangxiense). Of these, C. camelliae proved to be the most dominant and probably host specific taxon occurring on Camellia. An epitype is also designated for the latter species in this study. Colletotrichum jiangxiense is shown to be phylogenetically closely related to the coffee berry pathogen C. kahawae subsp. kahawae. Pathogenicity tests and the pairwise homoplasy index test suggest that C. jiangxiense and C. kahawae subsp. kahawae are two independent species. This study represents the first report of C. alienum and C. cliviae occurring on Camellia sinensis. In addition, our study demonstrated that the combined use of the loci ApMat and GS in a phylogenetic analysis is able to resolve all currently accepted species in the C. gloeosporioides species complex.
我们调查了来自中国七个省份（福建、贵州、河南、江西、四川、云南、浙江）的与茶树及其他山茶属植物有症状和无症状组织相关的144株炭疽菌分离株的系统发育多样性，以及从其他国家（包括印度尼西亚、英国和美国）获得的7株分离株。基于多位点（ACT、ApMat、CAL、GAPDH、GS、ITS、TUB2）系统发育分析和表型特征，区分出11个物种，包括9个特征明确的物种（异种炭疽菌、骨炭疽菌、茶炭疽菌、君子兰炭疽菌、菲奥里尼炭疽菌、果生炭疽菌、盘长孢炭疽菌、喀斯特炭疽菌、暹罗炭疽菌）和2个新物种（河南炭疽菌和江西炭疽菌）。其中，茶炭疽菌被证明是山茶属植物上最主要且可能具有寄主特异性的分类群。本研究还为后一个物种指定了一个后选模式。江西炭疽菌在系统发育上与咖啡浆果病原菌卡哈瓦炭疽菌卡哈瓦亚种密切相关。致病性测试和成对同塑性指数测试表明，江西炭疽菌和卡哈瓦炭疽菌卡哈瓦亚种是两个独立的物种。本研究首次报道了异种炭疽菌和君子兰炭疽菌在茶树上的出现。此外，我们的研究表明，在系统发育分析中联合使用ApMat和GS位点能够解析盘长孢炭疽菌种复合体内所有目前公认的物种。
