Lin Chia-Fa, Kuo Yen-Ting, Chen Tsung-Ying, Chien Chiang-Ting
Department of Life Science, College of Science, National Taiwan Normal University, Taipei 11677, Taiwan.
Department of Cardiology, Kuang-Tien General Hospital, Taichung 433, Taiwan.
Molecules. 2016 Mar 10;21(3):334. doi: 10.3390/molecules21030334.
We explored whether the combination of anti-oxidant and anti-inflammatory guava (Psidium guajava) and trehalose treatment protects the kidney and pancreas against Type II diabetes (T2DM)-induced injury in rats. We measured the active component of guava juice by HPLC analysis. T2DM was induced in Wistar rats by intraperitoneal administration of nicotinamide and streptozotocin and combination with high fructose diets for 8 weeks. The rats fed with different dosages of guava juice in combination with or without trehalose for 4 weeks were evaluated the parameters including OGTT, plasma insulin, HbA1c, HOMA-IR (insulin resistance) and HOMA-β (β cell function and insulin secretion). We measured oxidative and inflammatory degrees by immunohistochemistry stain, fluorescent stain, and western blot and serum and kidney reactive oxygen species (ROS) by a chemiluminescence analyzer. High content of quercetin in the guava juice scavenged H2O2 and HOCl, whereas trehalose selectively reduced H2O2, not HOCl. T2DM affected the levels in OGTT, plasma insulin, HbA1c, HOMA-IR and HOMA-β, whereas these T2DM-altered parameters, except HbA1c, were significantly improved by guava and trehalose treatment. The levels of T2DM-enhanced renal ROS, 4-hydroxynonenal, caspase-3/apoptosis, LC3-B/autophagy and IL-1β/pyroptosis were significantly decreased by guava juice and trehalose. The combination with trehalose and guava juice protects the pancreas and kidney against T2DM-induced injury.
我们探究了抗氧化和抗炎的番石榴（番石榴属）与海藻糖联合处理是否能保护大鼠的肾脏和胰腺免受II型糖尿病（T2DM）诱导的损伤。我们通过高效液相色谱分析测量了番石榴汁的活性成分。通过腹腔注射烟酰胺和链脲佐菌素并结合高果糖饮食8周，在Wistar大鼠中诱导T2DM。给大鼠喂食不同剂量的番石榴汁，联合或不联合海藻糖，持续4周，评估包括口服葡萄糖耐量试验（OGTT）、血浆胰岛素、糖化血红蛋白（HbA1c）、稳态模型评估胰岛素抵抗（HOMA-IR）和稳态模型评估β细胞功能（HOMA-β）等参数。我们通过免疫组织化学染色、荧光染色和蛋白质印迹法测量氧化和炎症程度，并通过化学发光分析仪测量血清和肾脏中的活性氧（ROS）。番石榴汁中高含量的槲皮素清除了过氧化氢（H2O2）和次氯酸（HOCl），而海藻糖选择性地降低了H2O2，而非HOCl。T2DM影响了OGTT、血浆胰岛素、HbA1c、HOMA-IR和HOMA-β的水平，而除HbA1c外，这些受T2DM改变的参数通过番石榴和海藻糖处理得到了显著改善。番石榴汁和海藻糖显著降低了T2DM增强的肾脏ROS、4-羟基壬烯醛、半胱天冬酶-3/凋亡、微管相关蛋白1轻链3β（LC3-B）/自噬和白细胞介素-1β/焦亡水平。海藻糖和番石榴汁联合处理可保护胰腺和肾脏免受T2DM诱导的损伤。
