Zeren Sezgin, Bayhan Zulfu, Koçak Cengiz, Koçak Fatma Emel, Metineren Mehmet Huseyin, Savran Bircan, Kocak Havva, Algin Mustafa Cem, Kahraman Cuneyt, Kocak Ahmet, Cosgun Suleyman
a Faculty of Medicine , Department of General Surgery , Dumlupinar University , Kutahya , Turkey.
b Faculty of Medicine , Department of Pathology , Dumlupinar University , Kutahya , Turkey.
J Invest Surg. 2017 Apr;30(2):116-124. doi: 10.1080/08941939.2016.1230247. Epub 2016 Oct 3.
Purpose/Aim: Oxidative stress plays an important role in the pathogenesis of acute pancreatitis (AP). We compared the therapeutic effects of Ukrain (NSC 631570) and N-acetylcysteine (NAC) in rats with AP.
Forty male Sprague Dawley rats were divided into four groups: controls; AP; AP with NAC; and AP with Ukrain. AP was induced via the ligation of the bile-pancreatic duct; drugs were administered intraperitoneally (i.p.) 30 min and 12 h after AP induction. Twenty-four hours after AP induction, animals were sacrificed and the pancreas was excised. Levels of malondialdehyde (MDA) and nitric oxide (NO), and activity levels of tumor necrosis factor (TNF)-α, and myeloperoxidase (MPO) were measured in tissue samples. Total oxidant status (TOS), total antioxidant status (TAS), and total bilirubin, as well as activity levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), amylase and lipase were measured in serum samples. Pancreatic tissue histopathology was also evaluated.
Test drugs reduced levels of MDA, NO, TNF-α, total bilirubin, AST, ALT, TOS and MPO, amylase and lipase activities (P < 0.001), and increased TAS (P < 0.001). Rats treated with test drugs attenuated AP-induced morphologic changes and decreased pancreatic damage scores compared with the AP group (P < 0.05). Both test drugs attenuated pancreatic damage, but the therapeutic effect was more pronounced in rats that received Ukrain than in those receiving NAC.
These results suggest that treatment with Ukrain or NAC can reduce pancreatic damage via anti-inflammatory and antioxidant effects.
目的/目标：氧化应激在急性胰腺炎（AP）的发病机制中起重要作用。我们比较了乌司他丁（NSC 631570）和N-乙酰半胱氨酸（NAC）对AP大鼠的治疗效果。
40只雄性Sprague Dawley大鼠分为四组：对照组；AP组；AP+NAC组；AP+乌司他丁组。通过结扎胆胰管诱导AP；在诱导AP后30分钟和12小时腹腔内注射药物。AP诱导24小时后，处死动物并切除胰腺。测定组织样本中丙二醛（MDA）、一氧化氮（NO）水平，肿瘤坏死因子（TNF）-α和髓过氧化物酶（MPO）的活性水平。测定血清样本中的总氧化剂状态（TOS）、总抗氧化剂状态（TAS）、总胆红素，以及天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、淀粉酶和脂肪酶的活性水平。还评估了胰腺组织的组织病理学。
受试药物降低了MDA、NO、TNF-α、总胆红素、AST、ALT、TOS和MPO、淀粉酶和脂肪酶的活性水平（P<0.001），并提高了TAS（P<0.001）。与AP组相比，受试药物治疗的大鼠减轻了AP诱导的形态学变化并降低了胰腺损伤评分（P<0.05）。两种受试药物均减轻了胰腺损伤，但乌司他丁治疗组大鼠的治疗效果比NAC治疗组更明显。
这些结果表明，乌司他丁或NAC治疗可通过抗炎和抗氧化作用减轻胰腺损伤。
