Chen Yingqing, Park Jeongmin, Joe Yeonsoo, Park Hyeok-Jun, Jekal Seung-Joo, Sato Daisuke, Hamada Hiroki, Chung Hun Taeg
Department of Biological Sciences, University of Ulsan, Ulsan 680-749, Republic of Korea.
Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan 570-750, Republic of Korea.
Oxid Med Cell Longev. 2017;2017:9427583. doi: 10.1155/2017/9427583. Epub 2017 May 7.
Pterostilbene, a dimethyl ester analog of resveratrol, has anti-inflammatory and antioxidative effects and alters cell proliferation. Tristetraprolin (TTP) promotes the degradation of proinflammatory mediators via binding to adenosine and uridine- (AU-) rich elements (ARE) located in the 3'-untranslated regions of mRNAs. Here, we utilized pterostilbene 4'--glucoside (4-PG), a compound derived from pterostilbene, to investigate whether it has anti-inflammatory effects on dextran sulfate sodium- (DSS-) induced colitis via TTP enhancement. TTP expression was increased in 4-PG dose- and time-dependent manners in RAW264.7 cells. The production of proinflammatory cytokine, such as TNF-, was reduced by 4-PG in vitro. To investigate the role of TTP in the anti-inflammatory effects of 4-PG, we used DSS-induced colitis in TTP WT and KO mice as models. The expression levels of TTP and proinflammatory cytokines were determined in serum and colon tissue. 4-PG increased the expression of TTP while suppressing proinflammatory cytokines both in vitro and in vivo. These findings suggest that treatment with 4-PG mediates the anti-inflammatory effects of 4-PG on DSS-induced colitis via enhancing TTP expression.
紫檀芪是白藜芦醇的二甲基酯类似物，具有抗炎和抗氧化作用，并能改变细胞增殖。Tristetraprolin（TTP）通过与位于mRNA 3'非翻译区的富含腺苷和尿苷（AU）的元件（ARE）结合，促进促炎介质的降解。在此，我们利用从紫檀芪衍生而来的化合物4'-O-葡萄糖苷紫檀芪（4-PG），来研究它是否通过增强TTP对硫酸葡聚糖钠（DSS）诱导的结肠炎具有抗炎作用。在RAW264.7细胞中，TTP的表达以4-PG剂量和时间依赖性方式增加。4-PG在体外可降低促炎细胞因子如TNF-α的产生。为了研究TTP在4-PG抗炎作用中的作用，我们以DSS诱导的TTP野生型和敲除型小鼠结肠炎为模型。测定血清和结肠组织中TTP和促炎细胞因子的表达水平。4-PG在体外和体内均增加TTP的表达，同时抑制促炎细胞因子。这些发现表明，4-PG处理通过增强TTP表达介导其对DSS诱导的结肠炎的抗炎作用。
