Poornejad Nafiseh, Buckmiller Evan, Schaumann Lara, Wang Haonan, Wisco Jonathan, Roeder Beverly, Reynolds Paul, Cook Alonzo
Department of Chemical Engineering, Brigham Young University, Provo, UT, USA.
Department of Genetics and Biotechnology, Brigham Young University, Provo, UT, USA.
J Tissue Eng. 2017 Jul 3;8:2041731417718809. doi: 10.1177/2041731417718809. eCollection 2017 Jan-Dec.
Decellularized porcine kidneys were recellularized with renal epithelial cells by three methods: perfusion through the vasculature under high pressure, perfusion through the ureter under high pressure, or perfusion through the ureter under moderate vacuum. Histology, scanning electron microscopy, confocal microscopy, and magnetic resonance imaging were used to assess vasculature preservation and the distribution of cells throughout the kidneys. Cells were detected in the magnetic resonance imaging by labeling them with iron oxide. Perfusion of cells through the ureter under moderate vacuum (40 mmHg) produced the most uniform distribution of cells throughout the kidneys.
高压下通过血管系统灌注、高压下通过输尿管灌注或中度真空下通过输尿管灌注。使用组织学、扫描电子显微镜、共聚焦显微镜和磁共振成像来评估血管系统的保存情况以及细胞在整个肾脏中的分布。通过用氧化铁标记细胞，在磁共振成像中检测到了细胞。在中度真空（40 mmHg）下通过输尿管灌注细胞在整个肾脏中产生了最均匀的细胞分布。
