用肾上皮细胞使整个猪肾重新上皮化。

Re-epithelialization of whole porcine kidneys with renal epithelial cells.

作者信息

Poornejad Nafiseh, Buckmiller Evan, Schaumann Lara, Wang Haonan, Wisco Jonathan, Roeder Beverly, Reynolds Paul, Cook Alonzo

机构信息

Department of Chemical Engineering, Brigham Young University, Provo, UT, USA.

Department of Genetics and Biotechnology, Brigham Young University, Provo, UT, USA.

出版信息

J Tissue Eng. 2017 Jul 3;8:2041731417718809. doi: 10.1177/2041731417718809. eCollection 2017 Jan-Dec.

DOI:10.1177/2041731417718809

PMID:28758007

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5513523/

Abstract

Decellularized porcine kidneys were recellularized with renal epithelial cells by three methods: perfusion through the vasculature under high pressure, perfusion through the ureter under high pressure, or perfusion through the ureter under moderate vacuum. Histology, scanning electron microscopy, confocal microscopy, and magnetic resonance imaging were used to assess vasculature preservation and the distribution of cells throughout the kidneys. Cells were detected in the magnetic resonance imaging by labeling them with iron oxide. Perfusion of cells through the ureter under moderate vacuum (40 mmHg) produced the most uniform distribution of cells throughout the kidneys.

摘要

通过三种方法将脱细胞猪肾用肾上皮细胞重新细胞化

高压下通过血管系统灌注、高压下通过输尿管灌注或中度真空下通过输尿管灌注。使用组织学、扫描电子显微镜、共聚焦显微镜和磁共振成像来评估血管系统的保存情况以及细胞在整个肾脏中的分布。通过用氧化铁标记细胞，在磁共振成像中检测到了细胞。在中度真空（40 mmHg）下通过输尿管灌注细胞在整个肾脏中产生了最均匀的细胞分布。