Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #09-01, Singapore, 138669, Singapore.
Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #09-01, Singapore, 138669, Singapore.
Biochimie. 2018 Feb;145:15-21. doi: 10.1016/j.biochi.2017.09.007. Epub 2017 Sep 13.
A novel aptamer generation method to greatly augment the affinity and stability of DNA aptamers was developed by genetic alphabet expansion combined with mini-hairpin DNA technology. The genetic alphabet expansion increases the physicochemical and structural diversities of DNA aptamers by introducing extra components, unnatural bases, as a fifth base, allowing for the enhancement of DNA aptamer affinities. Furthermore, the mini-hairpin DNA technology stabilizes DNA aptamers against nuclease digestion and thermal denaturation, by introducing an extraordinarily stable mini-hairpin DNA containing a GCGAAGC sequence. This novel method provides stabilized high-affinity DNA aptamers for diagnostic and therapeutic applications.
一种新的适体生成方法，通过遗传字母扩展结合迷你发夹 DNA 技术，极大地提高了 DNA 适体的亲和力和稳定性。遗传字母扩展通过引入额外的成分，非天然碱基，作为第五个碱基，增加了 DNA 适体的物理化学和结构多样性，从而提高了 DNA 适体的亲和力。此外，迷你发夹 DNA 技术通过引入含有 GCGAAGC 序列的极其稳定的迷你发夹 DNA，稳定了 DNA 适体免受核酸酶消化和热变性的影响。这种新方法为诊断和治疗应用提供了稳定的高亲和力 DNA 适体。
