Li Jiang, Wu Quan-Min, Wang Xiao-Qing, Zhang Cheng-Qiang
1 Department of Orthopedics, Shanghai Pudong New Area People's Hospital , Shanghai, China .
2 Department of Orthopedics, The Second People's Hospital of Dongying , Shandong, China .
DNA Cell Biol. 2017 Dec;36(12):1117-1125. doi: 10.1089/dna.2017.3888. Epub 2017 Oct 3.
Long noncoding RNAs (lncRNAs) have been illustrated to function as important regulator in carcinogenesis and cancer progression. However, roles of lncRNA miR210HG (miR210 host gene) in osteosarcoma remain unclear. In this study, miR210HG expression level was significantly upregulated in 55 cases of osteosarcoma tissue samples compared to adjacent normal tissue. Besides, the aberrantly enhanced miR210HG expression predicted poor prognosis and lower survival rate. In vitro, miR210HG knockdown suppressed the osteosarcoma cell proliferation, invasion, and epithelial-mesenchymal transition-related marker (N-cadherin and vimentin) expression. In vivo, miR210HG silencing decreased the tumor growth. miR-503 was verified to be the target miRNA of miR210HG using bioinformatics online program and luciferase assay. Furthermore, miR-503 could reverse the role of miR210HG on osteosarcoma cells. In conclusion, our study indicates that miR210HG sponges miR-503 to facilitate osteosarcoma cell invasion and metastasis, revealing the oncogenic role of miR210HG on osteosarcoma cells.
长链非编码RNA（lncRNAs）已被证明在肿瘤发生和癌症进展中发挥重要调节作用。然而，lncRNA miR210HG（miR210宿主基因）在骨肉瘤中的作用仍不清楚。在本研究中，与相邻正常组织相比，55例骨肉瘤组织样本中miR210HG表达水平显著上调。此外，miR210HG表达异常增强预示着预后不良和生存率较低。在体外，敲低miR210HG可抑制骨肉瘤细胞增殖、侵袭以及上皮-间质转化相关标志物（N-钙黏蛋白和波形蛋白）的表达。在体内，沉默miR210HG可降低肿瘤生长。使用生物信息学在线程序和荧光素酶测定法验证miR-503是miR210HG的靶标miRNA。此外，miR-503可逆转miR210HG对骨肉瘤细胞的作用。总之，我们的研究表明miR210HG通过吸附miR-503促进骨肉瘤细胞侵袭和转移，揭示了miR210HG在骨肉瘤细胞中的致癌作用。
