Dental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Sciences, King Saud University, Riyadh 11433, Saudi Arabia; Department of Oral Medicine and Radiology, KVG Dental College and Hospital, Sullia 574239, Karnataka, India.
Dental Department, Pediatric Dentist, Prince Sultan Military Medical City, Riyadh 11159, Saudi Arabia.
Int J Biol Macromol. 2018 Mar;108:790-797. doi: 10.1016/j.ijbiomac.2017.10.166. Epub 2017 Nov 1.
Various metal coated implants have been tested against dental pathogens which causes increased biofilm formation and lead to failure of dental implants. The possibility of using nanoparticle together with native biomolecules to enhance the activity of such bioactive compound is also in progress. In this study we tested the efficacy of Ag conjugated chitosan nanoparticles as a prospective coating material of titanium dental implants. Known bioactive molecule chitosan was extracted from A. flavus Af09 and conjugated with Ag nanoparticle. Fully characterized Ag-chitosan nanoparticle had a sound inhibitory effect on the growth of two major dental pathogens S. mutans and P. gingivalis. It not only inhibits the adhesion of these two tested bacteria but also able to reduce the biofilm formation. Apart from this, nanoparticle was also able to inhibit the QS production in bacteria tested in this study. Naturally extracted chitosan has been known for its antibacterial activity for a different group of bacteria. Nanoparticles are the good option to enhance the biocompatibility. No cell cytotoxicity of nanoparticle indicates its biocompatibility and coating of titanium dental implants with Ag-chitosan may have an added advantage on the corrosion resistance of dental implants and also augments the passivating effect of these implants.
各种金属涂层植入物已针对导致生物膜形成增加并导致牙科植入物失败的口腔病原体进行了测试。将纳米颗粒与天然生物分子一起使用以增强此类生物活性化合物的活性的可能性也在进行中。在这项研究中，我们测试了 Ag 结合壳聚糖纳米颗粒作为钛牙科植入物有前途的涂层材料的功效。从 A. flavus Af09 中提取出已知的生物活性分子壳聚糖，并与 Ag 纳米颗粒结合。经过充分表征的 Ag-壳聚糖纳米颗粒对两种主要口腔病原体 S. mutans 和 P. gingivalis 的生长具有良好的抑制作用。它不仅抑制了这两种测试细菌的粘附，而且还能够减少生物膜的形成。除此之外，纳米颗粒还能够抑制本研究中测试的细菌中 QS 的产生。天然提取的壳聚糖因其对不同细菌群的抗菌活性而闻名。纳米颗粒是增强生物相容性的好选择。纳米颗粒没有细胞毒性表明其生物相容性，并且用 Ag-壳聚糖涂覆钛牙科植入物可能对牙科植入物的耐腐蚀性具有附加优势，并且增强了这些植入物的钝化作用。
