研究 2 型糖尿病伴颈动脉粥样硬化患者 NLRP3 炎性体相关基因及其下游细胞因子。
Study of the NLRP3 inflammasome component genes and downstream cytokines in patients with type 2 diabetes mellitus with carotid atherosclerosis.
机构信息
Department of Clinical Laboratory, The second clinical medical college of yangtze university, Ren Min Road 1#, Jingzhou, Hubei, 434020, China.
Department of Endocrinology, The second clinical medical college of yangtze university, Jingzhou, China.
出版信息
Lipids Health Dis. 2017 Nov 18;16(1):217. doi: 10.1186/s12944-017-0595-2.
BACKGROUND
A role for the NLRP3 inflammasome has been reported in various diseases, such as diabetes mellitus, atherosclerosis (AS), nephropathy, rheumatism, and others, although limited information is available concerning the role of the NLRP3 inflammasome, interleukin-1β (IL-1β) and interleukin-18 (IL-18) in patients with type 2 diabetes mellitus (T2DM) and carotid atherosclerosis (CAS). Therefore, this cross-sectional study investigated these inflammatory components in patients with T2DM complicated with carotid atherosclerosis (T2DM + CAS).
METHODS
A total of 107 inpatients or outpatients were included,including 81 T2DM + CAS patients and 26 T2DM patients. Patients with T2DM or T2DM + CAS were recruited to compare the expression levels of NLRP3 pathway genes (NLRP3, ASC and caspase-1 mRNA) and the serum IL-1β and IL-18 concentrations. In the T2DM + CAS group, patients with thickened intima media thickness (IMT) and those with plaques were compared, and the correlation of the 5 variables with Crouse scores were analyzed.
RESULTS
The expression of NLRP3 pathway genes except caspase-1 was significantly higher in patients with T2DM and CAS compared to T2DM patients. Serum IL-1β and IL-18 concentrations shows no difference between the T2DM + CAS and T2DM group. In the T2DM + CAS group, the expression levels of the three inflammasome genes and IL-18 were increased in patients with thickened IMT compared to those with the plaque. All of the above factors negatively correlated with Crouse scores.
CONCLUSION
NLRP3 inflammasome pathway activity is significantly increased in patients with AS and T2DM at the early stage of plaque formation.
背景
NLRP3 炎性小体在各种疾病中发挥作用，如糖尿病、动脉粥样硬化（AS）、肾病、风湿病等，尽管有关 NLRP3 炎性小体、白细胞介素-1β（IL-1β）和白细胞介素-18（IL-18）在 2 型糖尿病（T2DM）和颈动脉粥样硬化（CAS）患者中的作用的信息有限。因此，本横断面研究调查了 T2DM 合并颈动脉粥样硬化（T2DM+CAS）患者的这些炎症成分。
方法
共纳入 107 例住院或门诊患者，包括 81 例 T2DM+CAS 患者和 26 例 T2DM 患者。招募 T2DM 或 T2DM+CAS 患者以比较 NLRP3 通路基因（NLRP3、ASC 和 caspase-1 mRNA）的表达水平以及血清 IL-1β 和 IL-18 浓度。在 T2DM+CAS 组中，比较了内膜中层厚度（IMT）增厚和斑块患者，并分析了 5 个变量与 Crouse 评分的相关性。
结果
与 T2DM 患者相比，T2DM 和 CAS 患者 NLRP3 通路基因（除 caspase-1 外）的表达明显升高。T2DM+CAS 组和 T2DM 组血清 IL-1β 和 IL-18 浓度无差异。在 T2DM+CAS 组中，与斑块患者相比，IMT 增厚患者三种炎性小体基因和 IL-18 的表达水平升高。所有这些因素均与 Crouse 评分呈负相关。
结论
在斑块形成的早期阶段，AS 和 T2DM 患者 NLRP3 炎性小体通路活性显著增加。
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