CD33 髓样细胞和 CD8 T 细胞浸润的 Merkel 细胞癌与改善的预后相关。
Merkel cell carcinomas infiltrated with CD33 myeloid cells and CD8 T cells are associated with improved outcome.
机构信息
Department of Pathology, Centre Hospitalier Universitaire (CHU) de Tours, Université Francois Rabelais, Tours, France; Biologie des Infections à Polyomavirus Team, Unité Mixte de Recherche 1282 Infectiologie et Santé Publique INRA, Universite Francois Rabelais, Tours, France; Department of Dermatology, Venereology and Allergology, University Hospital Würzburg, Würzburg, Germany.
Biologie des Infections à Polyomavirus Team, Unité Mixte de Recherche 1282 Infectiologie et Santé Publique INRA, Universite Francois Rabelais, Tours, France.
出版信息
J Am Acad Dermatol. 2018 May;78(5):973-982.e8. doi: 10.1016/j.jaad.2017.12.029. Epub 2017 Dec 19.
BACKGROUND
Merkel cell carcinoma (MCC) is a rare tumor of the skin that has an aggressive behavior. Immunity is the main regulator of MCC development, and many interactions between lymphocytes and tumor cells have been proven. However, the impact of tumor-infiltrating myeloid cells needs better characterization.
OBJECTIVE
To characterize tumor-infiltrating myeloid cells in MCC and their association with other immune effectors and patient outcome.
METHODS
MCC cases were reviewed from an ongoing prospective cohort study. In all, 103 triplicate tumor samples were included in a tissue microarray. Macrophages, neutrophils, and myeloid-derived suppressor cells were characterized by the following markers: CD68, CD33, CD163, CD15, CD33, and human leukocyte antigen-DR. Associations of these cell populations with programmed cell death ligand 1 expression, CD8 infiltrates, and vascular density were assessed. Impact on survival was analyzed by log-rank tests and a Cox multivariate model.
RESULTS
The median density of macrophages was 216 cells/mm. CD68 and CD33 macrophage densities were associated with CD8 T-cell infiltrates and programmed cell death ligand 1 expression. In addition, MCC harboring CD8 T cell infiltrates and brisk CD33 myeloid cell infiltrates were significantly and independently associated with improved outcomes (recurrence-free and overall survival).
LIMITATIONS
Sampling bias and the retrospective design were potential study limitations.
CONCLUSION
Infiltration of CD33 myeloid cells and CD8 T lymphocytes defines a subset of MCC associated with improved outcome.
背景
默克尔细胞癌（Merkel cell carcinoma，MCC）是一种侵袭性很强的皮肤肿瘤。免疫是 MCC 发展的主要调节因子，已经证明淋巴细胞与肿瘤细胞之间存在许多相互作用。然而，肿瘤浸润髓样细胞的影响需要更好地描述。
目的
描述 MCC 中的肿瘤浸润髓样细胞及其与其他免疫效应物和患者预后的关系。
方法
对一项正在进行的前瞻性队列研究中的 MCC 病例进行了回顾。总共纳入了 103 例 MCC 患者的 3 份肿瘤组织微阵列样本。通过以下标志物对巨噬细胞、中性粒细胞和髓源性抑制细胞进行特征描述：CD68、CD33、CD163、CD15、CD33 和人类白细胞抗原-DR。评估这些细胞群体与程序性细胞死亡配体 1 表达、CD8 浸润和血管密度的关系。通过对数秩检验和 Cox 多变量模型分析对生存的影响。
结果
巨噬细胞的中位密度为 216 个细胞/mm。CD68 和 CD33 巨噬细胞密度与 CD8 T 细胞浸润和程序性细胞死亡配体 1 表达相关。此外，MCC 中存在 CD8 T 细胞浸润和明显的 CD33 髓样细胞浸润与更好的预后（无复发生存和总生存）显著相关。
局限性
采样偏差和回顾性设计是潜在的研究局限性。
结论
CD33 髓样细胞和 CD8 T 淋巴细胞浸润定义了与改善预后相关的 MCC 亚群。
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