Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.
Curr Opin Struct Biol. 2018 Aug;51:129-134. doi: 10.1016/j.sbi.2018.03.014. Epub 2018 Apr 20.
Covalently circularized nanodiscs (cNDs) represent a significant advance in the durability and applicability of nanodisc technology. The new cNDs demonstrate higher size homogeneity and improved stability compared with that of non-circularized forms. Moreover, cNDs can be prepared at various defined sizes up to 80-nm diameter. The large cNDs can house much larger membrane proteins and their complexes than was previously possible with the conventional nanodiscs. In order to experience the full advantages of covalent circularization, high quality circularized scaffold protein and nanodisc samples are needed. Here, we give a concise overview and discuss the technical challenges that needed to be overcome in order to obtain high quality preparations. Furthermore, we review some potential new applications for the cNDs.
共价环化纳米盘 (cNDs) 代表了纳米盘技术在耐久性和适用性方面的重大进展。与非环化形式相比，新型 cNDs 具有更高的尺寸均一性和更好的稳定性。此外，cNDs 可以在各种定义的尺寸下制备，最大直径可达 80nm。较大的 cNDs 可以容纳比以前用传统纳米盘可能容纳的更大的膜蛋白及其复合物。为了充分体验共价环化的优势，需要高质量的环状支架蛋白和纳米盘样品。在这里，我们将简要概述并讨论为了获得高质量的制剂而需要克服的技术挑战。此外，我们还回顾了 cNDs 的一些潜在新应用。
