加拿大安大略省暴发流行性腮腺炎期间的实验室检测和系统发育分析。

Laboratory testing and phylogenetic analysis during a mumps outbreak in Ontario, Canada.

机构信息

Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada.

Public Health Ontario, 661 University Avenue, Toronto, Ontario, M5G 1M1, Canada.

出版信息

Virol J. 2018 Jun 5;15(1):98. doi: 10.1186/s12985-018-0996-5.

DOI:10.1186/s12985-018-0996-5

PMID:29866178

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5987625/

Abstract

BACKGROUND

In September 2009, a mumps outbreak originated in New York and spread to Northeastern USA and Canada. This study compares the performance of different diagnostic testing methods used in Ontario and describes molecular characteristics of the outbreak strain.

METHODS

Between September 2009 and February 2010, specimens from suspect cases were submitted to Public Health Ontario Laboratory for mumps serology, culture and/or real-time reverse-transcriptase PCR (rRT-PCR) testing. rRT-PCR-positive specimens underwent genotyping at Canada's National Microbiology Laboratory. Whole genome sequencing was performed on four outbreak and three sporadic viral culture isolates.

RESULTS

Six hundred ninety-eight patients had IgM serology testing, of which 255 (37%) had culture and rRT-PCR. Among those, 35/698 (5%) were IgM positive, 39/255 (15%) culture positive and 47/255 (18%) rRT-PCR-positive. Buccal swabs had the highest rRT-PCR positivity (21%). The outbreak isolates were identical to that in the New York outbreak occurring at the same time. Nucleotide and amino acid identity with the Jeryl Lynn vaccine strain ranged from 85.0-94.5% and 82.4-99.4%, depending on the gene and coding sequences. Homology of the HN protein, the main immunogenic mumps virus protein, was found to be 94.5 and 95.3%, when compared to Jeryl Lynn vaccine major and minor components, respectively.

CONCLUSIONS

Despite higher sensitivity than serology, rRT-PCR testing is underutilized. Further work is needed to better understand the suboptimal match of the HN gene between the outbreak strain and the Jeryl Lynn vaccine strain.

摘要

背景

2009 年 9 月，一场腮腺炎疫情起源于纽约，并蔓延至美国东北部和加拿大。本研究比较了安大略省使用的不同诊断检测方法的性能，并描述了疫情毒株的分子特征。

方法

2009 年 9 月至 2010 年 2 月期间，公共卫生安大略省实验室收到了疑似病例的标本，用于腮腺炎血清学、培养和/或实时逆转录聚合酶链反应（rRT-PCR）检测。rRT-PCR 阳性标本在加拿大国家微生物学实验室进行基因分型。对 4 个疫情和 3 个散发性病毒培养分离株进行了全基因组测序。

结果

698 例患者进行了 IgM 血清学检测，其中 255 例（37%）进行了培养和 rRT-PCR 检测。在这些患者中，698 例中有 35 例（5%）IgM 阳性，255 例中有 39 例（15%）培养阳性，255 例中有 47 例（18%）rRT-PCR 阳性。口腔拭子的 rRT-PCR 阳性率最高（21%）。疫情分离株与同时发生的纽约疫情分离株完全相同。与 Jeryl Lynn 疫苗株的核苷酸和氨基酸同一性范围分别为 85.0-94.5%和 82.4-99.4%，取决于基因和编码序列。与 Jeryl Lynn 疫苗的主要和次要成分相比，HN 蛋白的同源性分别为 94.5%和 95.3%，HN 蛋白是主要的免疫性腮腺炎病毒蛋白。