Li G Q, Liu F F, Li J Q, Liu Q L, Chen S F
China Eucalypt Research Centre (CERC), Chinese Academy of Forestry (CAF), ZhanJiang 524022, GuangDong Province, China.
Persoonia. 2018 Dec;40:63-95. doi: 10.3767/persoonia.2018.40.03. Epub 2017 Oct 31.
The is a species-rich family that includes pathogens of a wide variety of plants, including species of . Recently, during disease surveys in China, diseased samples associated with species of were collected from plantation and other plants, including , , and , which were growing adjacent to . In addition, few samples from and in two gardens were also included in this study. Disease symptoms observed mainly included stem canker, shoot and twig blight. In this study, 105 isolates of were collected from six provinces, of which 81 isolates were from trees. These isolates were identified based on comparisons of the DNA sequences of the internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS), and partial translation elongation factor 1-alpha (), β-tubulin (), DNA-directed RNA polymerase II subunit () and calmodulin () genes, the nuclear ribosomal large subunit (LSU) and the nuclear ribosomal small subunit (SSU), and combined with their morphological characteristics. Results showed that these isolates represent 12 species of , including , , , , and , and six previously undescribed species of and , namely sp. nov., sp. nov., sp. nov., sp. nov., sp. nov. and sp. nov. Aside from , and , the other nine species were isolated from trees in South China. (26 % of the isolates from ) is the dominant species, followed by (23 % of the isolates from ). In addition to species found on trees, we also found on ; on ; on ; on , and ; and on . Pathogenicity tests showed that the 12 species of are pathogenic to three clones and that species are the most aggressive. The results of our study suggest that many more species of the remain to be discovered in China. This study also provides confirmation for the wide host range of species on different plants.
这是一个物种丰富的科，包括多种植物的病原体，其中包括……的物种。最近，在中国进行病害调查期间，从种植园……以及与……相邻生长的其他植物，包括……、……、……和……中收集了与……物种相关的患病样本。此外，本研究还纳入了来自两个花园中……和……的少量样本。观察到的病害症状主要包括茎溃疡、枝梢枯萎。在本研究中，从六个省份收集了105株……分离株，其中81株分离自……树。这些分离株基于内部转录间隔区和居间的5.8S nrRNA基因（ITS）、部分翻译延伸因子1-α（……）、β-微管蛋白（……）、DNA指导的RNA聚合酶II亚基（……）和钙调蛋白（……）基因、核糖体大亚基（LSU）和核糖体小亚基（SSU）的DNA序列比较，并结合其形态特征进行鉴定。结果表明，这些分离株代表了……的12个物种，包括……、……、……、……、……和……，以及六个先前未描述的……和……物种，即……新种、……新种、……新种、……新种、……新种和……新种。除了……、……和……之外，其他九个……物种是从中国南方的……树上分离得到的。……（占分离自……的分离株的26%）是优势种，其次是……（占分离自……的分离株的23%）。除了在……树上发现的物种外，我们还在……上发现了……；在……上发现了……；在……上发现了……；在……、……和……上发现了……；在……上发现了……。致病性测试表明，……的这12个物种对三个……克隆具有致病性，并且……物种最具侵袭性。我们的研究结果表明，在中国仍有更多的……物种有待发现。本研究还证实了……物种对不同植物具有广泛的寄主范围。
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