São Paulo State University (Unesp), School of Pharmaceutical Sciences, Department of Clinical Analysis, Araraquara, São Paulo, Brazil.
Graduate Program in Biomedical Sciences Hermínio Ometto University Center, UNIARARAS, 7 Av. Dr. Maximiliano Baruto, 500, CEP 13607-339, Araras, SP.
Phytother Res. 2019 Apr;33(4):976-988. doi: 10.1002/ptr.6291. Epub 2019 Jan 18.
Insulin with natural antioxidants is emerging as a combination treatment for diabetes mellitus that attempts to exert effective glycemic control without adverse effects. The present study aimed to investigate the additive effects on metabolic disturbances, oxidative damage, and antioxidant defenses in streptozotocin-diabetic rats treated with curcumin and a reduced insulin dose. The best results were obtained in the treatment of diabetic rats with 4-U/day insulin; however, the glycemia levels in these rats were lower than those in normal rats, indicating a risk of hypoglycemia. Isolated treatments using curcumin or insulin in a reduced dose (1 U/day) decreased glycemia, dyslipidemia, and biomarkers of liver and kidney damage and increased the activity of hepatic antioxidants (superoxide dismutase and glutathione peroxidase), however, only to a lesser extent than 4-U/day insulin, without improvements in catalase activity or plasma lipid peroxidation. Decreases in glycemia, dyslipidemia, and tissue damage markers were more evident in the curcumin + 1-U/day insulin treatment than those seen in isolated treatments. The activity of hepatic antioxidants, including catalase, was further increased, and biomarkers of oxidative damage were decreased. Curcumin with a reduced insulin dose appears to be a promising strategy for combating the complications associated with diabetes and oxidative stress.
具有天然抗氧化剂的胰岛素作为一种联合治疗糖尿病的方法正在出现，它试图在没有不良反应的情况下发挥有效的血糖控制作用。本研究旨在研究姜黄素和降低剂量胰岛素治疗链脲佐菌素诱导的糖尿病大鼠对代谢紊乱、氧化损伤和抗氧化防御的附加作用。每天 4U 胰岛素治疗糖尿病大鼠的效果最佳；然而，这些大鼠的血糖水平低于正常大鼠，表明有发生低血糖的风险。用姜黄素或胰岛素（每天 1U）进行单独治疗可降低血糖、血脂、肝和肾损伤的生物标志物，并增加肝抗氧化剂（超氧化物歧化酶和谷胱甘肽过氧化物酶）的活性，但程度低于每天 4U 胰岛素，且对过氧化氢酶活性或血浆脂质过氧化没有改善作用。与单独治疗相比，姜黄素+每天 1U 胰岛素治疗在降低血糖、血脂和组织损伤标志物方面更为明显。肝抗氧化剂的活性，包括过氧化氢酶，进一步增加，氧化损伤的生物标志物减少。用降低剂量的胰岛素联合姜黄素似乎是一种有前途的策略，可以对抗糖尿病和氧化应激相关的并发症。
