Liu Jiushi, Wei Xueping, Zhang Xiaoyi, Qi Yaodong, Zhang Bengang, Liu Haitao, Xiao Peigen
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Beijing, China.
Front Pharmacol. 2019 Jan 22;9:1576. doi: 10.3389/fphar.2018.01576. eCollection 2018.
The stems and roots of species have been used as the folk medicine in Traditional Chinese medicine (TCM) and have good traditional efficacy and medicinal application with a long history. Among these species, , and are the most widely distributed species in the regions of south and southwest China. Owing to their similar appearance, the crude drugs are often confusedly used by some folk doctors, even some pharmaceutical factories. To discriminate the crude drugs, haplotype analysis based on cpDNA markers and ITS was firstly employed in this study. Generic delimitation, interspecific interrelationships, and the identification of medicinal materials between and remained unresolved by the existing molecular fragments. The original plant could be identified through the morphological character of flower, fruit and leaf. However, in most situation collectors have no chance to find out these characters due to lack of reproductive organs, and have no experience with the minor difference and transitional variation of leaf morphology. The chemical characterization show that the chemometric of chemical composition owned higher resolution to discriminate three herbs of species. In conclusion, this integrative approach involving molecular phylogeny, morphology and chemical characterization could be applied for authentication of the . Our study suggests the use of this comprehensive approach for accurate characterization of this closely related taxa as well as identifying the source plant and confused herbs of TCM.
该物种的茎和根在中国传统医学（TCM）中一直被用作民间药物，具有良好的传统疗效和药用应用历史悠久。在这些物种中，[具体物种名称1]、[具体物种名称2]和[具体物种名称3]是中国南方和西南地区分布最广的物种。由于它们外观相似，一些民间医生甚至一些制药厂经常混淆使用这些药材。为了鉴别这些药材，本研究首次采用基于cpDNA标记和ITS的单倍型分析。现有分子片段尚未解决[具体属名]的属级界定、种间亲缘关系以及[具体物种名称1]和[具体物种名称2]之间药材的鉴定问题。原植物可以通过花、果实和叶的形态特征来鉴定。然而，在大多数情况下，采集者由于缺乏生殖器官而没有机会发现这些特征，并且对叶形态的细微差异和过渡变异也没有经验。化学特征表明，化学成分的化学计量学对鉴别[具体物种名称1]的三种草药具有更高的分辨率。总之，这种涉及分子系统发育、形态学和化学特征的综合方法可用于[具体物种名称1]的鉴定。我们的研究建议使用这种综合方法来准确表征这种密切相关的分类群，以及识别中药的源植物和混淆草药。
