微波辅助一步法合成明胶固载的聚乙二醇荧光碳点作为高效的甲氨蝶呤递药载体。
Microwave-assisted and one-step synthesis of PEG passivated fluorescent carbon dots from gelatin as an efficient nanocarrier for methotrexate delivery.
机构信息
a Faculty of Chemistry, Department of Organic and Biochemistry, Polymer Research Laboratory , University of Tabriz , Tabriz , Iran.
b Department of Chemical Engineering , University of South Carolina , Columbia , SC , USA.
出版信息
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):540-547. doi: 10.1080/21691401.2018.1562460.
A green and simple process for preparing the polyethylene glycol passivated fluorescent carbon dots (CDs-PEG) have been studied by a microwave pyrolysis method, using gelatin and PEG as starting materials. This method is very effective for development of carbon-based quantum dots from gelatin with high quantum yield (QY). The synthesized CDs-PEG were found to emit blue photoluminescence (PL) with a maximum QY of 34%. At the following research, we investigated the effect of the presence of PEG on PL intensity, and the result showed that CDs-PEG becomes stronger PL properties than pure CDs from gelatin. The synthesized CDs-PEG were characterized by FTIR, TEM, UV-vis, PL, zeta potential and XRD analyses. The anticancer performance of developed CDs-PEG was evaluated by in vitro tests such as MTT assay and fluorescence microscopy analyses. The examination of CDs-PEG as an anti-cancer drug nanocarrier for methotrexate (MTX) illustrated a better antitumor efficacy than free MTX due to its enhanced nuclear delivery in vitro, which resulting in highly effective tumour growth inhibition and improving targeted cancer therapy in clinical medicine.
一种绿色、简单的方法，通过微波热解法，以明胶和 PEG 为起始原料，制备了聚乙二醇修饰的荧光碳点（CDs-PEG）。该方法非常有效地从明胶中开发出具有高量子产率（QY）的碳量子点。合成的 CDs-PEG 发蓝色光致发光（PL），QY 高达 34%。在随后的研究中，我们研究了 PEG 的存在对 PL 强度的影响，结果表明，PEG 修饰的 CDs-PEG 比纯 CDs-PEG 具有更强的 PL 性质。通过傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、紫外-可见吸收光谱（UV-vis）、PL、Zeta 电位和 X 射线衍射（XRD）分析对合成的 CDs-PEG 进行了表征。通过 MTT 分析和荧光显微镜分析等体外试验评价了所开发的 CDs-PEG 的抗癌性能。作为甲氨蝶呤（MTX）的抗癌药物纳米载体的研究表明，由于其在体外增强了核内传递，与游离 MTX 相比，具有更好的抗肿瘤疗效，从而高度有效地抑制肿瘤生长，改善临床肿瘤靶向治疗。
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