1Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, College of Medicine, Seoul National University, 20 Boramae-ro 5-gil, Dongjak-gu, Seoul, 07061 Korea.
2Department of Translational Medicine, College of Medicine, Seoul National University, Daehak-ro 103, Jongno-gu, Seoul, 03080 Korea.
Tissue Eng Regen Med. 2019 Mar 4;16(2):151-159. doi: 10.1007/s13770-019-00182-2. eCollection 2019 Apr.
Unlike bone, cartilage, or muscle, tendon-specific markers are not well established. The purpose of the study was to investigate expression pattern and level of 6 well-known tendon-specific markers, in various human musculoskeletal tissues, tenocytes, and mesenchymal stem cells (MSCs).
Musculoskeletal tissue samples of tendon, bone, cartilage, nerve, muscle, and fat were obtained from patients undergoing orthopedic surgery. Tenocytes, MSCs from bone marrow, adipose tissue, and umbilical cord were isolated from each tissue and cultured. Six tendon-specific markers, scleraxis (Scx), tenomodulin (TNMD), thrombospondin-4 (TSP-4), tenascin-C (TNC), type I collagen (Col I), and type III collagen (Col III) were investigated in tendon tissue, tenocytes, and MSCs.
mRNA levels of 6 tendon-specific markers were significantly higher in tendon tissue that in other connective tissues levels of Scx, TNMD, TSP-4, and Col III immediately decreased after plating tenocytes in culture dishes whereas those of TNC and Col I did not. In comparison with tendon tissue, mRNA levels pattern of Scx, TNMD, and TSP-4 in tenocytes were significantly higher than that in MSCs, but lower than in tendon tissue whereas expression pattern of TNC, Col I and III showed different pattern with each other.
This study demonstrated that 6 commonly used tendon-specific markers were mainly expressed in tendon tissue, but that expression level and pattern of the tendon-specific markers with respect to kinds of tissues, culture duration of tenocytes and sources of MSCs.
与骨骼、软骨或肌肉不同，肌腱特异性标志物尚未得到很好的建立。本研究旨在研究 6 种已知的肌腱特异性标志物在各种人体肌肉骨骼组织、肌腱细胞和间充质干细胞（MSCs）中的表达模式和水平。
从接受骨科手术的患者中获得肌腱、骨骼、软骨、神经、肌肉和脂肪等肌肉骨骼组织样本。从每种组织中分离出肌腱细胞和骨髓、脂肪组织和脐带 MSC，并进行培养。研究了 6 种肌腱特异性标志物，包括硬皮素（Scx）、腱调蛋白（TNMD）、血小板反应蛋白-4（TSP-4）、腱糖蛋白 C（TNC）、I 型胶原（Col I）和 III 型胶原（Col III）在肌腱组织、肌腱细胞和 MSCs 中的表达。
6 种肌腱特异性标志物的 mRNA 水平在肌腱组织中明显高于其他结缔组织；肌腱细胞在培养皿中接种后，Scx、TNMD、TSP-4 和 Col III 的水平立即下降，而 TNC 和 Col I 的水平则没有下降。与肌腱组织相比，Scx、TNMD 和 TSP-4 在肌腱细胞中的 mRNA 水平模式明显高于 MSCs，但低于肌腱组织；而 TNC、Col I 和 Col III 的表达模式彼此不同。
本研究表明，6 种常用的肌腱特异性标志物主要在肌腱组织中表达，但表达水平和模式与组织种类、肌腱细胞培养时间和 MSCs 来源有关。
