1Department of Biomedical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Jalal ale Ahmad Highway, P.O. Box 14115-114, Tehran, Iran.
2Tissue Engineering and Applied Cell Sciences Division, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Jalal ale Ahmad Highway, P.O. Box 14115-331, Tehran, Iran.
Tissue Eng Regen Med. 2019 Mar 21;16(3):237-251. doi: 10.1007/s13770-019-00184-0. eCollection 2019 Jun.
BACKGROUND: Nowadays, production of nanocomposite scaffolds based on natural biopolymer, bioceramic, and metal ions is a growing field of research due to the potential for bone tissue engineering applications. METHODS: In this study, a nanocomposite scaffold for bone tissue engineering was successfully prepared using collagen (COL), beta-tricalcium phosphate (β-TCP) and strontium oxide (SrO). A composition of β-TCP (4.9 g) was prepared by doping with SrO (0.05 g). Biocompatible porous nanocomposite scaffolds were prepared by freeze-drying in different formulations [COL, COL/β-TCP (1:2 w/w), and COL/β-TCP-Sr (1:2 w/w)] to be used as a provisional matrix or scaffold for bone tissue engineering. The nanoparticles were characterized by X-ray diffraction, Fourier transforms infrared spectroscopy and energy dispersive spectroscopy. Moreover, the prepared scaffolds were characterized by physicochemical properties, such as porosity, swelling ratio, biodegradation, mechanical properties, and biomineralization. RESULTS: All the scaffolds had a microporous structure with high porosity (~ 95-99%) and appropriate pore size (100-200 μm). COL/β-TCP-Sr scaffolds had the compressive modulus (213.44 ± 0.47 kPa) higher than that of COL/β-TCP (33.14 ± 1.77 kPa). cytocompatibility, cell attachment and alkaline phosphatase (ALP) activity studies performed using rat bone marrow mesenchymal stem cells. Addition of β-TCP-Sr to collagen scaffolds increased ALP activity by 1.33-1.79 and 2.92-4.57 folds after 7 and 14 days of culture, respectively. CONCLUSION: In summary, it was found that the incorporation of Sr into the collagen-β-TCP scaffolds has a great potential for bone tissue engineering applications.
背景：如今，基于天然生物聚合物、生物陶瓷和金属离子的纳米复合材料支架的生产因其在骨组织工程应用中的潜力而成为一个日益增长的研究领域。
方法：在这项研究中，成功地使用胶原蛋白（COL）、β-磷酸三钙（β-TCP）和氧化锶（SrO）制备了用于骨组织工程的纳米复合材料支架。通过掺杂 SrO（0.05 g）制备了组成为β-TCP（4.9 g）的纳米复合材料支架。通过在不同配方中冷冻干燥（COL、COL/β-TCP（1:2 w/w）和 COL/β-TCP-Sr（1:2 w/w））来制备生物相容性多孔纳米复合材料支架，用作临时基质或骨组织工程支架。通过 X 射线衍射、傅里叶变换红外光谱和能谱对纳米粒子进行了表征。此外，还对制备的支架进行了物理化学性质的表征，如孔隙率、溶胀比、生物降解性、力学性能和生物矿化。
结果：所有支架均具有微孔结构，具有高孔隙率（~95-99%）和适当的孔径（100-200 μm）。COL/β-TCP-Sr 支架的压缩模量（213.44 ± 0.47 kPa）高于 COL/β-TCP（33.14 ± 1.77 kPa）。通过大鼠骨髓间充质干细胞进行细胞相容性、细胞附着和碱性磷酸酶（ALP）活性研究。在 COL 支架中加入β-TCP-Sr 后，第 7 天和第 14 天培养后 ALP 活性分别提高了 1.33-1.79 倍和 2.92-4.57 倍。
结论：综上所述，发现将 Sr 掺入胶原-β-TCP 支架中具有很大的骨组织工程应用潜力。
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