Division of Pathogen Genomics, Translational Genomics Research Institute-North, Flagstaff, AZ, USA; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA; Faculty of Medicine, University of Brasília, Brasília-DF, Brazil.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Fungal Genet Biol. 2020 May;138:103351. doi: 10.1016/j.fgb.2020.103351. Epub 2020 Feb 3.
Modern genome analysis and phylogenomic methods have increased the number of fungal species, as well as enhanced appreciation of the degree of diversity within the fungal kingdom. In this context, we describe a new Parengyodontium species, P. americanum, which is phylogenetically related to the opportunistic human fungal pathogen P. album. Five unusual fungal isolates were recovered from five unique and confirmed coccidioidomycosis patients, and these isolates were subsequently submitted to detailed molecular and morphological identification procedures to determine identity. Molecular and morphological diagnostic analyses showed that the isolates belong to the Cordycipitaceae. Subsequently, three representative genomes were sequenced and annotated, and a new species, P. americanum, was identified. Using various genomic analyses, gene family expansions related to novel compounds and potential for ability to grow in diverse habitats are predicted. A general description of the genomic composition of this newly described species and comparison of genome content with Beauveria bassiana, Isaria fumosorosea and Cordyceps militaris shows a shared core genome of 6371 genes, and 148 genes that appear to be specific for P. americanum. This work provides the framework for future investigations of this interesting fungal species.
现代基因组分析和系统发育基因组学方法增加了真菌物种的数量，同时也提高了对真菌界多样性程度的认识。在此背景下，我们描述了一种新的拟青霉属物种，即美洲拟青霉，它与机会性人类真菌病原体球毛壳霉具有系统发育关系。从五个独特且确诊的球孢子菌病患者中分离出五个不寻常的真菌分离株，随后对这些分离株进行详细的分子和形态学鉴定程序，以确定其身份。分子和形态学诊断分析表明，这些分离株属于虫草科。随后，对三个有代表性的基因组进行测序和注释，确定了一个新物种，即美洲拟青霉。通过各种基因组分析，预测到与新型化合物相关的基因家族扩张和在不同生境中生长的潜力。对这个新描述的物种的基因组组成进行了一般描述，并与球孢白僵菌、玫烟色棒束孢和蛹虫草的基因组内容进行比较，显示出一个共享的 6371 个基因的核心基因组，以及 148 个似乎是美洲拟青霉特有的基因。这项工作为进一步研究这个有趣的真菌物种提供了框架。
