Mendeleum-Institute of Genetics, Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic.
Laboratoire de Biologie des Systèmes Microbiens (LBSM), Département des Sciences Naturelles, Ecole Normale Supérieure de Kouba-Alger, Alger, Algeria.
PLoS One. 2020 May 20;15(5):e0232448. doi: 10.1371/journal.pone.0232448. eCollection 2020.
Several Botryosphaeriaceae species are known to occur worldwide, causing dieback, canker and fruit rot on various hosts. Surveys conducted in ten commercial citrus orchards in the northern region of Algeria revealed five species of Botryosphaeriaceae belonging to three genera associated with diseased trees. Morphological and cultural characteristics as well as phylogenetic analyses of the internal transcribed spacer (ITS) region and the translation elongation factor 1-alpha (tef1-α) identified Diplodia mutila, Diplodia seriata, Dothiorella viticola, Lasiodiplodia mediterranea and a novel species which is here described as Lasiodiplodia mithidjana sp. nov.. Of these, L. mithidjana (14.1% of the samples) and L. mediterranea (13% of the samples) were the most widespread and abundant species. Pathogenicity tests revealed that L. mediterranea and D. seriata were the most aggressive species on citrus shoots. This study highlights the importance of Botryosphaeriaceae species as agents of canker and dieback of citrus trees in Algeria.
已发现几种 Botryosphaeriaceae 物种在世界范围内存在，它们会导致各种宿主的枯枝病、溃疡病和果实腐烂。在阿尔及利亚北部的十个商业柑橘园进行的调查显示，有五种 Botryosphaeriaceae 物种属于三个属，与患病树木有关。形态学和文化特征以及内部转录间隔区（ITS）区域和翻译延伸因子 1-α（tef1-α）的系统发育分析鉴定出 Diplodia mutila、Diplodia seriata、Dothiorella viticola、Lasiodiplodia mediterranea 和一种新物种，即本文描述的 Lasiodiplodia mithidjana sp. nov.。在这些物种中，L. mithidjana（占样本的 14.1%）和 L. mediterranea（占样本的 13%）分布最广且数量最多。致病性试验表明，L. mediterranea 和 D. seriata 是柑橘嫩枝上最具侵袭性的物种。本研究强调了 Botryosphaeriaceae 物种作为阿尔及利亚柑橘枯梢和枯枝病的病原菌的重要性。
