Cox G W, Orosz C G, Lewis M G, Olsen R G, Fertel R H
Department of Pharmacology, College of Medicine, Ohio State University, Columbus 43210.
Int J Immunopharmacol. 1988;10(6):773-81. doi: 10.1016/0192-0561(88)90031-8.
This study was designed to characterize the effects of the anti-psoriatic compound 8-methoxypsoralen (8-MOP) on human lymphocyte function in vitro. Normal human peripheral blood mononuclear cells were stimulated with an optimal (1%) or a suboptimal (0.05%) concentration of phytohemagglutinin (PHA). At the optimal concentration of PHA, 8-MOP (140 microM) caused a delay in lymphocyte proliferation, interleukin-2 (IL-2) production/accumulation and IL-2 receptor expression. Addition of exogenous IL-2 to cultures stimulated with an optimal concentration of PHA did not overcome the delay of lymphocyte proliferation and IL-2 receptor expression. At the suboptimal concentration of PHA, 8-MOP (140 microM) caused a sustained inhibition of lymphocyte proliferation, IL-2 production/accumulation and IL-2 receptor expression. Addition of exogenous IL-2 under these conditions restored the magnitude of lymphocyte proliferation and IL-2 receptor expression. However, the responses displayed the delayed lymphocyte proliferation and IL-2 receptor expression typical of cells incubated with 8-MOP and an optimal concentration of PHA.
本研究旨在表征抗银屑病化合物8-甲氧基补骨脂素（8-MOP）对体外人淋巴细胞功能的影响。用最佳浓度（1%）或次最佳浓度（0.05%）的植物血凝素（PHA）刺激正常人外周血单个核细胞。在PHA的最佳浓度下，8-MOP（140微摩尔）导致淋巴细胞增殖、白细胞介素-2（IL-2）产生/积累和IL-2受体表达延迟。向用最佳浓度PHA刺激的培养物中添加外源性IL-2并不能克服淋巴细胞增殖和IL-2受体表达的延迟。在PHA的次最佳浓度下，8-MOP（140微摩尔）导致淋巴细胞增殖、IL-2产生/积累和IL-2受体表达持续受到抑制。在这些条件下添加外源性IL-2可恢复淋巴细胞增殖的幅度和IL-2受体表达。然而，这些反应表现出与用8-MOP和最佳浓度PHA孵育的细胞典型的淋巴细胞增殖延迟和IL-2受体表达。
