利用桃金娘叶提取物和丝胶合成生物源银纳米粒子的抗菌局部凝胶的潜力。
Potential of antimicrobial topical gel with synthesized biogenic silver nanoparticle using Rhodomyrtus tomentosa leaf extract and silk sericin.
机构信息
Cosmetic Technology and Dietary Supplement Products Program, Faculty of Agro and Bio Industry, Thaksin University, Ban Pa Phayom, Phatthalung, 93210, Thailand.
Division of Infectious Diseases, Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
出版信息
Biotechnol Lett. 2020 Dec;42(12):2653-2664. doi: 10.1007/s10529-020-02971-5. Epub 2020 Jul 18.
OBJECTIVE
Silver nanoparticles synthesized using Rhodomyrtus tomentosa leaf extract and silk sericin were used to functionalize carbopol 940 gel for topical applications.
RESULTS
UV-vis spectra presented surface plasmon resonance at 426 nm, transmission electron microscopy revealed that nanoparticles were spherical with an average size of 25-50 nm. X-ray diffraction presented crystalline silver nanoparticles with zeta potential of ≈ - 30 mV. FTIR spectra showed a reduction of silver nitrate indicated by the shift in -OH at 2958 cm. The silver nanoparticle demonstrated broad-spectrum antimicrobial activity against Gram-positive, Gram-negative and fungi with MIC ranging between 0.26 and 2.10 μg mL, respectively. MIC of hydrogel ranged between 1.05-2.10 μg mL with cell viability of 89%. Spreadability and extrudability of gel were 9.3 ± 0.85 s and 19.85 ± 0.03%, respectively with first order of fickian diffusion.
CONCLUSIONS
The silver nanoparticle gel exhibited an effective antimicrobial property, hence can be exploited for topical applications.
目的
使用皱叶酸模叶提取物和丝胶蛋白合成的银纳米粒子被用于对卡波姆 940 凝胶进行功能化，以用于局部应用。
结果
紫外-可见光谱在 426nm 处呈现出表面等离子体共振，透射电子显微镜显示纳米粒子为球形，平均粒径为 25-50nm。X 射线衍射呈现出结晶态的银纳米粒子，其 ζ 电位约为-30mV。傅里叶变换红外光谱显示硝酸银被还原，-OH 在 2958cm 处发生位移。银纳米粒子对革兰氏阳性菌、革兰氏阴性菌和真菌表现出广谱抗菌活性，其 MIC 分别在 0.26 至 2.10μg/mL 之间。水凝胶的 MIC 介于 1.05-2.10μg/mL 之间，细胞活力为 89%。凝胶的铺展性和挤出性分别为 9.3±0.85s 和 19.85±0.03%，符合一级菲克扩散。
结论
银纳米粒子凝胶表现出有效的抗菌性能，因此可用于局部应用。
Zotero 插件