抗阻训练和姜黄补充剂对糖尿病大鼠活性物质标志物应激的影响。

Effects of resistance training and turmeric supplementation on reactive species marker stress in diabetic rats.

作者信息

Júnior Ailton Santos Sena, Aidar Felipe José, Santos Jymmys Lopes Dos, Estevam Charles Dos Santos, Dos Santos Jessica Denielle Matos, de Oliveira E Silva Ana Mara, Lima Fábio Bessa, De Araújo Silvan Silva, Marçal Anderson Carlos

机构信息

Department of Physical Education, Universidade Federal de Sergipe, São Cristóvão, Sergipe Brazil.

Group of Studies and Research of Performance, Sport, Health and Paralympic Sports - GEPEPS, Universidade Federal de Sergipe, São Cristóvão, Sergipe Brazil.

出版信息

BMC Sports Sci Med Rehabil. 2020 Aug 6;12:45. doi: 10.1186/s13102-020-00194-9. eCollection 2020.

DOI:10.1186/s13102-020-00194-9

PMID:32774865

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7409633/

Abstract

BACKGROUND

Type 1 (T1DM) is a metabolic disease characterized by hyperglycemia and excessive generation of reactive oxygen species caused by autoimmune destruction of beta-cells in the pancreas. Among the antioxidant compounds, (CL) has potential antioxidant effects and may improve hyperglycemia in uncontrolled T1DM/TD1, as well as prevent its complications (higher costs for the maintenance of health per patient, functional disability, cardiovascular disease, and metabolic damage). In addition to the use of compounds to attenuate the effects triggered by diabetes, physical exercise is also essential for glycemic control and the maintenance of skeletal muscles. Our objective is to evaluate the effects of CL supplementation associated with moderate- to high-intensity resistance training on the parameters of body weight recovery, glycemic control, reactive species markers, and tissue damage in rats with T1DM/TD1.

METHODS

Forty male 3-month-old Wistar rats (200-250 g) with alloxan-induced T1DM were divided into 4 groups ( = 7-10): sedentary diabetics (DC); diabetic rats that underwent a 4-week resistance training protocol (TD); CL-supplemented diabetic rats (200 mg/kg body weight, 3x a week) (SD); and supplemented diabetic rats under the same conditions as above and submitted to training (TSD). Body weight, blood glucose, and the following biochemical markers were analyzed: lipid profile, aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid, creatine kinase (CK), lactate dehydrogenase (LDH), and thiobarbituric acid reactive substances (TBARS).

RESULTS

Compared to the DC group, the TD group showed body weight gain (↑7.99%, = 0.0153) and attenuated glycemia (↓23.14%, = 0.0008) and total cholesterol (↓31.72%, ≤ 0.0041) associated with diminished reactive species markers in pancreatic (↓45.53%, < 0.0001) and cardiac tissues (↓51.85%, < 0.0001). In addition, compared to DC, TSD promoted body weight recovery (↑15.44%, ≤ 0.0001); attenuated glycemia (↓42.40%, 0.0001), triglycerides (↓39.96%, ≤ 0.001), and total cholesterol (↓28.61%, ≤ 0.05); and attenuated the reactive species markers in the serum (↓26.92%, ≤ 0.01), pancreas (↓46.22%, ≤ 0.0001), cardiac (↓55.33%, ≤ 0.001), and skeletal muscle (↓42.27%, ≤ 0.001) tissues caused by T1DM.

CONCLUSION

Resistance training associated (and/or not) with the use of attenuated weight loss, the hypoglycemic and hypolipidemic effects, reactive species markers, and T1DM-induced tissue injury.

摘要

背景

1型糖尿病（T1DM）是一种代谢性疾病，其特征为高血糖以及胰腺中β细胞因自身免疫破坏而导致活性氧过度生成。在抗氧化化合物中，肉桂酸（CL）具有潜在的抗氧化作用，可能改善未控制的T1DM/TD1中的高血糖，并预防其并发症（每位患者维持健康的成本更高、功能残疾、心血管疾病和代谢损伤）。除了使用化合物来减轻糖尿病引发的影响外，体育锻炼对于血糖控制和骨骼肌维持也至关重要。我们的目的是评估CL补充剂与中高强度抗阻训练相结合对T1DM/TD1大鼠体重恢复、血糖控制、活性物质标志物和组织损伤参数的影响。

方法

将40只3月龄、体重200 - 250克、用四氧嘧啶诱导T1DM的雄性Wistar大鼠分为4组（每组n = 7 - 10）：久坐不动的糖尿病大鼠（DC）；接受为期4周抗阻训练方案的糖尿病大鼠（TD）；补充CL的糖尿病大鼠（200毫克/千克体重，每周3次）（SD）；以及在上述相同条件下补充CL并接受训练的糖尿病大鼠（TSD）。分析体重、血糖以及以下生化标志物：血脂谱、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、尿酸、肌酸激酶（CK）、乳酸脱氢酶（LDH）和硫代巴比妥酸反应性物质（TBARS）。

结果

与DC组相比，TD组体重增加（↑7.99%，P = 0.0153），血糖降低（↓23.14%，P = 0.0008），总胆固醇降低（↓31.72%，P ≤ 0.0041），同时胰腺（↓45.53%，P < 0.0001）和心脏组织（↓51.85%，P < 0.0001）中的活性物质标志物减少。此外，与DC组相比，TSD组促进了体重恢复（↑15.44%，P ≤ 0.0001）；降低了血糖（↓42.40%，P = 0.0001）、甘油三酯（↓39.96%，P ≤ 0.001）和总胆固醇（↓28.61%，P ≤ 0.05）；并减轻了T1DM导致的血清（↓26.92%，P ≤ 0.01）、胰腺（↓46.22%，P ≤ 0.0001）、心脏（↓55.33%，P ≤ 0.001）和骨骼肌（↓42.27%，P ≤ 0.001）组织中的活性物质标志物。