台湾生物银行参与者中 PM 暴露与 DLEC1 启动子甲基化。
PM exposure and DLEC1 promoter methylation in Taiwan Biobank participants.
机构信息
Institute of Medicine, Chung Shan Medical University, Taichung City, 40201, Taiwan.
School of Medical Imaging and Radiological Sciences, Chung Shan Medical University, Taichung City, 40201, Taiwan.
出版信息
Environ Health Prev Med. 2020 Nov 5;25(1):68. doi: 10.1186/s12199-020-00909-x.
BACKGROUND
Particulate matter (PM) < 2.5 μm (PM) or fine PM is a serious public health concern. It affects DNA methylation and heightens carcinogenesis. Deleted in lung and esophageal cancer 1 (DLEC1) is a tumor suppressor gene. However, aberrant methylation of the gene is associated with several cancers. We evaluated the association between PM and DLEC1 promoter methylation in Taiwanese adults based on regular outdoor exercise.
METHODS
We obtained DNA methylation and exercise data of 496 participants (aged between 30 and 70 years) from the Taiwan Biobank (TWB) database. We also extracted PM data from the Air Quality Monitoring Database (AQMD) and estimated participants' exposure using residential addresses.
RESULTS
DLEC1 methylation and PM were positively associated: beta coefficient (β) = 0.114 × 10; p value = 0.046. The test for interaction between exercise and PM on DLEC1 methylation was significant (p value = 0.036). After stratification by exercise habits, PM and DLEC1 methylation remained significantly associated only among those who exercised regularly (β = 0.237 × 10; p value = 0.007). PM quartile-stratified analyses revealed an inverse association between regular exercise and DLEC1 methylation at PM < 27.37 μg/m (β = - 5.280 × 10; p value = 0.009). After combining exercise habits and PM quartiles, one stratum (i.e., regular exercise and PM < 27.37 μg/m) was inversely associated with DLEC1 methylation (β = -5.160 × 10, p value = 0.007).
CONCLUSIONS
We found significant positive associations between PM and DLEC1 promoter methylation. Regular exercise at PM < 27.37 μg/m seemingly regulated DLEC1 promoter methylation.
背景
颗粒物（PM）<2.5μm（PM）或细颗粒物是一个严重的公共卫生问题。它会影响 DNA 甲基化并加剧致癌作用。缺失于肺癌和食管癌 1 号基因（DLEC1）是一种肿瘤抑制基因。然而，基因的异常甲基化与多种癌症有关。我们根据台湾成年人的常规户外锻炼，评估了 PM 与 DLEC1 启动子甲基化之间的关系。
方法
我们从台湾生物银行（TWB）数据库中获得了 496 名参与者（年龄在 30 岁至 70 岁之间）的 DNA 甲基化和运动数据。我们还从空气质量监测数据库（AQMD）中提取了 PM 数据，并使用居住地址来估计参与者的暴露情况。
结果
DLEC1 甲基化与 PM 呈正相关：β系数（β）=0.114×10；p 值=0.046。PM 对 DLEC1 甲基化的交互作用检验具有统计学意义（p 值=0.036）。根据运动习惯进行分层后，仅在经常运动的人群中，PM 和 DLEC1 甲基化仍呈显著相关（β=0.237×10；p 值=0.007）。PM 四分位分层分析显示，经常运动与 PM<27.37μg/m 时 DLEC1 甲基化呈负相关（β=-5.280×10；p 值=0.009）。在结合运动习惯和 PM 四分位数后，一个亚组（即经常运动且 PM<27.37μg/m）与 DLEC1 甲基化呈负相关（β=-5.160×10，p 值=0.007）。
结论
我们发现 PM 与 DLEC1 启动子甲基化之间存在显著的正相关关系。PM<27.37μg/m 时的规律运动似乎调节了 DLEC1 启动子甲基化。
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