不是基质血管成分(SVF)或纳米脂肪,而是总基质细胞(TOST):新定义。机械性基质细胞提取技术的系统综述。
Not Stromal Vascular Fraction (SVF) or Nanofat, but Total Stromal-Cells (TOST): A New Definition. Systemic Review of Mechanical Stromal-Cell Extraction Techniques.
机构信息
Plastic and Reconstructive Surgery, MEST Medical Services, Cumhuriyet Bulv. No:161/A,1,2 Alsancak, Izmir, Turkey.
出版信息
Tissue Eng Regen Med. 2021 Feb;18(1):25-36. doi: 10.1007/s13770-020-00313-0. Epub 2020 Nov 24.
The most important and greatest source in the body for regenerative cells is fat tissue. Obtaining regenerative cells from adipose tissue can be done in two ways: Enzymatic and mechanical. The regenerative cell cocktail obtained by the enzymatic method, including stem cells, is called Stromal vascular fracture (SVF). In the literature, there is no clear definition of regenerative cells obtained by mechanical method. We systematically searched the techniques and definitions for stromal cells obtained from adipose tissue by scanning different databases. To evaluate the mechanical stromal-cell isolation techniques and end products from adipose tissue. Systematic review of English and non-English articles using Embase, PubMed, Web of Science and Google scholar databases. Search terms included Nanofat, fragmented fat, mechanical stromal / stem cell, mechanical SVF, SVF gel. We screened all peer-reviewed articles related with mechanical stromal-cell isolation. Author performed a literature query with the aforementioned key words and databases. A total of 276 publications containing the keywords we searched were reached. In these publications, there are 46 different definitions used to obtain mechanical stromal cells. The term SVF is only suitable for enzymatic methods. A different definition is required for mechanical. The most used term nanofat is also not suitable because the product is not in both "fat" and in "nanoscale". We think that the term total stromal-cells would be the most appropriate definition since both extracellular matrix and all stromal cells are protected in mechanical methods.
人体内最重要和最丰富的再生细胞来源是脂肪组织。从脂肪组织中获取再生细胞有两种方法:酶法和机械法。酶法获得的再生细胞混合物,包括干细胞,称为基质血管片段(SVF)。在文献中,机械法获得的再生细胞没有明确的定义。我们系统地扫描了不同的数据库,以获取脂肪组织中基质细胞的技术和定义。为了评估从脂肪组织中机械分离基质细胞的技术和终产物。对英文和非英文文献进行了系统评价,使用了 Embase、PubMed、Web of Science 和 Google Scholar 数据库。搜索词包括纳米脂肪、碎片脂肪、机械基质/干细胞、机械 SVF、SVF 凝胶。我们筛选了所有与机械基质细胞分离相关的同行评议文章。作者使用上述关键词和数据库进行了文献查询。共检索到 276 篇包含我们搜索的关键词的出版物。在这些出版物中,有 46 种不同的定义用于获得机械基质细胞。SVF 一词仅适用于酶法。机械法需要不同的定义。最常用的术语纳米脂肪也不适用,因为产品既不在“脂肪”中,也不在“纳米级”中。我们认为,术语“总基质细胞”是最合适的定义,因为在机械方法中,细胞外基质和所有基质细胞都得到了保护。