柴胡-龙骨-牡蛎汤通过改善海马神经元细胞焦亡发挥抗癫痫作用。
Chaihu-Longgu-Muli Decoction exerts an antiepileptic effect in rats by improving pyroptosis in hippocampal neurons.
机构信息
Provincial Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan Province, 410208, China; Key Laboratory of TCM Heart and Lung Syndrome Differentiation ＆ Medicated Diet and Dietotherapy, Hunan University of Chinese Medicine, Changsha, Hunan Province, 410208, China.
Department of Psychiatry, Hunan Brain Hospital, Clinical Medical School, Hunan University of Chinese Medicine, Changsha, Hunan Province, 410007, China.
出版信息
J Ethnopharmacol. 2021 Apr 24;270:113794. doi: 10.1016/j.jep.2021.113794. Epub 2021 Jan 7.
ETHNOPHARMACOLOGICAL RELEVANCE
Chaihu-Longgu-Muli Decoction (CLMD) is a classic prescription created by Zhong-jing Zhang, a famous ancient Chinese medical scientist, to harmonize uncontrollable body activities and calm the minds. Now Traditional Chinese Medicine (TCM) physicians often apply it to treat psychiatric diseases such as epilepsy.
AIM OF THE STUDY
This study investigated the mechanism of the effect of Chaihu-Longgu-Muli Decoction (CLMD) on hippocampal neurons pyroptosis in rats with Temporal Lobe Epilepsy (TLE).
MATERIALS AND METHODS
The lithium chloride-pilocarpine-induced TLE rat model was established. The behavioral testing was performed and, the expression of IL-1β and TNF-α in serum was detected by ELISA, qRT-PCR was used to detect the mRNA expression of NLRP3, Caspase-1, IL-1β and TNF-α in hippocampus. The expression of NLRP3 and Caspase-1 in hippocampal dentate gyrus was detected by immunofluorescence assay.
RESULTS
CLMD could significantly suppress the frequency and duration time of epileptic seizures, reduce the expression of NLRP3, Caspase-1 TNF-α and IL-1β.
CONCLUSIONS
CLMD exerted an obvious antiepileptic effect by improving pyroptosis in hippocampal neurons of TLE rats.
民族药理学相关性
柴胡龙骨牡蛎汤（CLMD）是由中国古代著名医学科学家张仲景创立的经典方剂，用于调和失控的身体活动和镇静心灵。现在，中医（TCM）医生经常用它来治疗癫痫等精神疾病。
研究目的
本研究旨在探讨柴胡龙骨牡蛎汤（CLMD）对颞叶癫痫（TLE）大鼠海马神经元细胞焦亡的作用机制。
材料和方法
建立氯化锂-匹罗卡品诱导的 TLE 大鼠模型。通过行为测试，ELISA 检测血清中 IL-1β 和 TNF-α 的表达，qRT-PCR 检测海马中 NLRP3、Caspase-1、IL-1β 和 TNF-α 的 mRNA 表达。免疫荧光法检测海马齿状回 NLRP3 和 Caspase-1 的表达。
结果
CLMD 能显著抑制癫痫发作的频率和持续时间，降低 NLRP3、Caspase-1、TNF-α 和 IL-1β 的表达。
结论
CLMD 通过改善 TLE 大鼠海马神经元细胞的细胞焦亡，发挥明显的抗癫痫作用。
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