Murray A, Crocker P R, Wood R F
Professorial Surgical Unit, St Bartholomew's Hospital, London, UK.
Br J Surg. 1988 Apr;75(4):349-51. doi: 10.1002/bjs.1800750418.
The use of a pulsed dye laser to ablate atheromatous tissue obtained from post-mortem human aortic specimens is reported. Laser energy was delivered with a 600 micron quartz fibre, at a wavelength of 504 nm and a pulse length of 1 microseconds. Pulse energy was varied from 30-140 mJ, producing peak pulse powers of the order of 100 kW. With these parameters the laser ablated fatty, fibrous and calcified plaques. At this wavelength atheroma is vaporized but there is minimal damage to normal vessel wall, due to preferential absorption of the laser light. Light microscopy shows that by microsecond pulsing, thermal damage to surrounding tissues associated with continuous wave lasers is avoided. Transmission electron micrographs reveal a sharp demarcation between a laser crater and the adjacent vessel wall with little ultrastructural disruption. Scanning electron micrographs show the crater walls to be smooth. The pulsed dye laser may therefore be effective in the treatment of occlusive peripheral vascular disease without undue risk of vessel perforation.
据报道，使用脉冲染料激光消融从尸检人体主动脉标本中获取的动脉粥样硬化组织。激光能量通过一根600微米的石英纤维传递，波长为504纳米，脉冲长度为1微秒。脉冲能量在30 - 140毫焦之间变化，产生的峰值脉冲功率约为100千瓦。在这些参数下，激光能够消融脂肪、纤维和钙化斑块。在这个波长下，动脉粥样硬化组织被汽化，但由于激光的优先吸收，对正常血管壁的损伤最小。光学显微镜显示，通过微秒脉冲，可以避免连续波激光对周围组织造成的热损伤。透射电子显微镜照片显示激光坑与相邻血管壁之间有明显的界限，超微结构破坏很小。扫描电子显微镜照片显示坑壁光滑。因此，脉冲染料激光在治疗闭塞性外周血管疾病时可能有效，且不会有过度的血管穿孔风险。
